Basicity of the amino groups of the aminoglycoside amikacin using capillary electrophoresis and coupled CE-MS-MS techniques.
Basicity of the amino groups of the aminoglycoside amikacin using capillary electrophoresis and coupled CE-MS-MS techniques.
复制标题
使用毛细管电泳和耦合 CE-MS-MS 技术测定氨基糖苷阿米卡星氨基的碱度。
DOI：
10.1021/ac010173m
复制
发表时间：
2001
影响因子：
7.4
通讯作者：
Whitesides,GM
中科院分区：
文献类型：
--
作者：
Kane,RS;Glink,PT;Chapman,RG;McDonald,JC;Jensen,PK;Gao,H;Pasa-Tolić,L;Smith,RD;Whitesides,GM
本文介绍了用毛细管电泳（CE）及CE与质谱联用技术测定氨基糖苷类抗生素阿米卡星及其乙酰化衍生物的氨基pKa值。这些pKa值（8.4，6.7，9.7，8.4）是通过测量分子的电泳迁移率作为pH值的函数来确定的;它们与文献中报道的某些值（通过13 C和15 N NMR光谱）在0.7个单位内，但解决了这些早期研究留下的模糊性。氨基的pKa值的范围也表明了官能团的酸度（以及在特定pH值下的电离程度）对该基团的分子环境的复杂依赖性。
This paper describes the use of capillary electrophoresis (CE), and coupled CE and mass spectrometric techniques, to measure the values of the pKaof the amino groups of the aminoglycoside antibiotic amikacin and of its acetylated derivatives. These values of pKa(8.4, 6.7, 9.7, 8.4) were determined by measuring the electrophoretic mobilities of the molecules as a function of pH; they are within 0.7 unit of certain values reported in the literature (by13C and15N NMR spectroscopies) but resolved ambiguities left by these earlier studies. The range of values of pKaof amino groups also indicates the complex dependence of the acidity of a functional group (and thus the extent of ionization at a specified value of pH) on the molecular environment of that group.