Gold Nanoparticles-Coated SU-8 for Sensitive Fluorescence-Based Detections of DNA
Gold Nanoparticles-Coated SU-8 for Sensitive Fluorescence-Based Detections of DNA
复制标题
金纳米颗粒涂层 SU-8 用于基于荧光的 DNA 灵敏检测
作者：
C. Cao;S. Birtwell;Jonas Høgberg;H. Morgan;A. Wolff;D. D. Bang
SU-8环氧基负光刻胶已被广泛用作制造许多生物微机电系统（Bio-MEMS）或片上实验室（LOC）器件的结构材料。然而，SU-8具有较高的自身荧光水平，限制了使用荧光作为主要检测手段的微设备的灵敏度。在这里，我们发现在SU-8表面沉积一层薄薄的金纳米颗粒层，与裸露的SU-8相比，涂层SU-8表面的自身荧光显著降低了81%。此外，DNA探针可以很容易地固定在金表面，具有很高的热稳定性。这些改进使得通过简单的DNA杂交低至1 nM（两个数量级的改进）或通过具有亚皮摩尔灵敏度的固相PCR进行敏感的DNA检测成为可能。该方法简单且易于执行，适用于使用SU-8作为结构材料的各种Bio-MEMs和LOC器件。
SU-8 epoxy-based negative photoresist has been extensively employed as a structural material for fabrication of numerous biological microelectro-mechanical systems (Bio-MEMS) or lab-on-a-chip (LOC) devices. However, SU-8 has a high autofluorescence level that limits sensitivity of microdevices that use fluorescence as the predominant detection workhorse. Here, we show that deposition of a thin gold nanoparticles layer onto the SU-8 surface significantly reduces the autofluorescence of the coated SU-8 surface by as much as 81% compared to bare SU-8. Furthermore, DNA probes can easily be immobilized on the Au surface with high thermal stability. These improvements enabled sensitive DNA detection by simple DNA hybridization down to 1 nM (a two orders of magnitude improvement) or by solid-phase PCR with sub-picomolar sensitivity. The approach is simple and easy to perform, making it suitable for various Bio-MEMs and LOC devices that use SU-8 as a structural material.
影响因子：
38.3
作者：
You, Chang-Cheng;Miranda, Oscar R.;Rotello, Vincent M.
通讯作者：
Rotello, Vincent M.