Pharmacokinetic comparison of gossypol isomers in cattle: transfer from diet to plasma and degradation by rumen microbes
Pharmacokinetic comparison of gossypol isomers in cattle: transfer from diet to plasma and degradation by rumen microbes
复制标题
牛中棉酚异构体的药代动力学比较：从饮食转移到血浆并被瘤胃微生物降解
DOI：
10.1631/jzus.b1700289
复制
发表时间：
2018-06-01
影响因子：
5.1
通讯作者：
Zhang, Jun-min
中科院分区：
文献类型：
--
作者：
Tang, Chao-hua;Liu, Jia;Zhang, Jun-min
通过研究棉酚异构体从摄入的食物到血浆的转移以及瘤胃微生物的降解，对牛体内的棉酚异构体进行了药代动力学比较。采用高效液相色谱 (HPLC) 测定全棉籽、血浆和瘤胃液中的棉酚异构体。三个农场的奶牛对(+)-和(-)-棉酚的摄入量分别约为5.6-8.5和3.8-5.9克/(d中心点群)。血浆棉酚浓度随着摄入量的增加而增加，(+)形式的血浆棉酚浓度为0.31至0.48μg/ml，(-)形式的血浆棉酚浓度为0.39至0.59μg/ml。奶牛摄入的棉酚中，(+) 形式稍占优势（58.8%-59.8%），而（-）形式在血浆中占优势（54.6%-55.9%）。体外研究表明，6小时时，500和1000μg/g处理组的(-)-棉酚分别降解了67.4%和85.7%，12小时时分别增加到83.6%和92.5%。 (+)-棉酚的降解规律与(-)-棉酚相似。这些结果表明，(-)-棉酚在血浆中可能比(+)-棉酚更持久，并且棉酚异构体在瘤胃中的降解很快并且在牛中不具有对映选择性。
A pharmacokinetic comparison of gossypol isomers in cattle was made by investigating their transfer from ingested food to plasma and their degradation by rumen microbes. The gossypol isomers in whole cottonseed, plasma, and rumen fluid were determined by high-performance liquid chromatography (HPLC). The intakes of (+)- and (-)- gossypol by cows on three farms were about 5.6-8.5 and 3.8-5.9 g/(d center dot herd), respectively. The plasma gossypol concentrations increased as ingestion increased and ranged from 0.31 to 0.48 mu g/ml for the (+) form and from 0.39 to 0.59 mu g/ml for the (-) form. The (+) form was slightly predominant (58.8%-59.8%) in the gossypol ingested by the cows, whereas the (-) form predominated (54.6%-55.9%) in the plasma. An in vitro study showed that at 6 h, 67.4% and 85.7% of the (-)-gossypol were degraded in 500 and 1000 mu g/g treatment groups, respectively, and these increased to 83.6% and 92.5%, respectively, at 12 h. The regularity of the degradation of (+)-gossypol was similar to that of (-)-gossypol. These results showed that (-)-gossypol may be more persistent than (+)-gossypol in plasma, and that the degradation of the gossypol isomers in the rumen is rapid and not enantioselective in cattle.