Changes in collagen cross-linking in degenerative disc disease and scoliosis
Changes in collagen cross-linking in degenerative disc disease and scoliosis
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DOI：
10.1097/00007632-199812010-00009
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发表时间：
1998-12-01
期刊：
影响因子：
3
通讯作者：
Roberts, S
中科院分区：
文献类型：
--
作者：
Duance, VC;Crean, JKG;Roberts, S
研究设计.对手术中从椎间盘退变或脊柱侧凸相关腰痛患者中收集的人椎间盘胶原交联特征变化的生物化学研究。确定与椎间盘退变或脊柱侧凸相关的腰痛患者椎间盘中胶原交联特征是否发生变化，这可能会影响基质完整性。组织中的这种交联情况表明基质更新和组织重塑增加，可能对这些疾病的进展有影响。椎间盘疾病、退行性椎间盘疾病和脊柱侧凸的特征都是细胞外基质成分的变化，这将影响组织的机械功能。胶原成分的稳定性以及因此结缔组织如椎间盘的机械完整性取决于胶原分子之间交联的程度和类型。本文报道了椎间盘中不同交联的分布以及随着年龄、退行性椎间盘疾病和脊柱侧凸而发生的变化。33个椎间盘来自退行性椎间盘疾病患者，29个椎间盘来自脊柱侧凸患者。样品酸水解和胶原交联分析的任何分馏上的氨基酸分析仪配置为使用茚三酮柱后检测交联分析或分馏的高压液相色谱法与荧光检测。可还原交联和成熟交联均从外纤维环向髓核内增加。在软骨终板中发现了最高水平的成熟交联。相比之下，非酶衍生的交联戊糖苷在椎间盘上几乎没有差异，但确实显示出预期的年龄相关性增加。在退行性椎间盘疾病中，未发现可还原或成熟交联水平的变化，但在更严重的疾病样本中观察到年龄相关交联戊糖苷水平的降低。在脊柱侧凸中，在脊柱侧凸椎间盘的凸侧比凹侧发现了显著更高水平的可还原交联。在退行性椎间盘疾病和脊柱侧凸中椎间盘的交联轮廓的这些变化指示增加的基质转换和组织重塑，并且可能对这些疾病的进展具有影响。
Study Design. Biochemical study of the changes in the collagen cross-link profile of human intervertebral discs collected at surgery from patients with either low back pain associated with disc degeneration or scoliosis.Objective. To determine whether changes occur in the collagen cross-link profile in the disc of patients with either low back pain associated with disc degeneration or scoliosis, which may well influence matrix integrity. Such chances in the cross-link profile of a tissue indicates increased matrix turnover and tissue remodeling and may have implications for the progression of these disorders.Summary of Background Data. The diseases of the intervertebral disc, degenerative disc disease and scoliosis, are both characterized by changes in the extracellular matrix components that will affect the mechanical function of the tissue. The stability of the collagenous components and hence the mechanical integrity of connective tissues such as the disc is dependent on the degree and type of cross-links between the collagen molecules. This article reports results on the distribution of the different cross-links in the disc and the changes that occur with age, degenerative disc disease, and scoliosis.Methods. Thirty-three discs were obtained from patients with degenerative disc disease and 29 discs from patients with scoliosis. Samples were acid hydrolyzed and the collagen cross-links analyzed by either fractionation on an amino acid analyzer configured for cross-link analysis using ninhydrin postcolumn detection or fractionation by high-pressure liquid chromatography with fluorescence detection.Results. The reducible cross-links and the mature cross-link all increased from the outer anulus fibrosus through into the nucleus pulposus. The highest levels of the mature cross-link were found in the cartilage end-plate. The nonenzymic derived cross-link, pentosidine, in contrast, showed little difference across the disc, but did show the expected age-related increase. In degenerative disc disease, no change in the levels of the reducible or mature cross-links was found, but a decrease was observed in the levels of the age-related cross-link pentosidine in the more severe disease samples. In scoliosis, significantly higher levels of the reducible cross-links were found on the convex than on the concave side of the scoliotic disc.Conclusions. These changes in the cross-link profile of the intervertebral disc in degenerative disc disease and scoliosis are indicative of increased matrix turnover and tissue remodeling and likely to have implications for the progression of these disorders.