Phylogeny of the order Scytosiphonales (Phaeophyceae) based on DNA sequences of rbcL, partial rbcS, and partial LSU nrDNA
Phylogeny of the order Scytosiphonales (Phaeophyceae) based on DNA sequences of rbcL, partial rbcS, and partial LSU nrDNA
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DOI：
10.2216/i0031-8884-38-6-496.1
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发表时间：
1999-11-01
期刊：
影响因子：
1.6
通讯作者：
Masuda, M
中科院分区：
文献类型：
--
作者：
Kogame, K;Horiguchi, T;Masuda, M
利用14个种的rbcL、部分r-BCS和部分大亚基nrDNA(结构域D1和D2)的DNA序列，对裸藻亚目(褐藻目)的分子系统发育进行了研究。采用Etocarpus silulosus(Dillwyn)Lyngbai作为外类群。在最大简约性和邻接性分析中推断的系统发育树几乎彼此相同，并显示出两个大的分支。分布于暖温带至热带地区的一个单系类群包括Chnoospora implexa J.Agardh、Rosenvingea Intricata(J.Agardh)Borgesen、水生枝状水生孢子虫Howe和Colpomeia sinuosa(Merten Ex Roth)Derbes et Solier。第二类群包括Colpomeia Bullosa(Saunders)Yamada，Colpomeia phaeodactyla Wynne et J.Norris，Scell siphon的4种，以及花瓣属的3种，主要分布于寒温带地区。Peregrina Colpomeia peregrina(Sauvageau)Hamel在两个大支系之间表现出了模棱两可的立场。在第二大支系中，花瓣吸管亚支、花瓣吸管吸管亚支和纤细管吸管吸管亚支以及另一支纤细管吸管吸管亚支和结节花瓣吸管吸管亚支都得到了较高的自助值支持。这些结果严重地质疑了花孢科的自主性，以及花瓣属、粘虫属和花瓣属的单系性。平卧孢子体的形态特征，如菌体结构和有无多眼的带状丝虫，与分子系统学一致。这些特征可能是比直立配子体的形态更重要的属或科水平的分类标准。
A molecular phylogenetic study of the order Scytosiphonales (Phaeophyceae) was carried out using DNA sequences of rbcL, partial r-bcS, and partial large subunit (LSU) nrDNA (domains D1 and D2) that were determined in 14 species. Ectocarpus siliculosus (Dillwyn) Lyngbye was adopted as an outgroup taxon. Phylogenetic trees inferred in maximum-parsimony and neighbor-joining analyses were almost identical to each other and showed two large clades. A monophyletic group distributed in warm-temperate to tropical regions includes Chnoospora implexa J. Agardh, Rosenvingea intricata (J. Agardh) Borgesen, Hydroclatrus clathratus (C. Agardh) Howe, and Colpomenia sinuosa (Mertens ex Roth) Derbes et Solier. The second clade includes Colpomenia bullosa (Saunders) Yamada, Colpomenia phaeodactyla Wynne et J. Norris, four species of Scytosiphon, and three species of Petalonia, which are mostly distributed in cold-temperate regions. Colpomenia peregrina (Sauvageau) Hamel showed an ambiguous position between the two large clades. Within the second large clade, a subclade of Petalonia fascia (O. F. Muller) Kuntze, P. binghamiae (J. Agardh) Vinogradova, and Scytosiphon tenellus Kogame and another subclade of Scytosiphon gracilis Kogame and Petalonia zosterifolia (Reinke) Kuntze were supported by high bootstrap values. These results seriously question autonomy of the Chnoosporaceae and monophyly of the genera Colpomenia, Scytosiphon, and Petalonia. Morphological characters of the prostrate sporophytes, such as thallus structure and presence or absence of plurilocular zoidangia, were congruent with the molecular phylogeny. These features are likely to be more important taxonomic criteria at the generic or family level in the Scytosiphonales than is the morphology of the erect gametophytes.