Ferredoxin:NADP+ Oxidoreductase, is a Subunit of the Cytochrome b6f Complex of Spinach Chloroplasts: Implications for the Pathway of Cyclic Electron Transport
Ferredoxin:NADP+ Oxidoreductase, is a Subunit of the Cytochrome b6f Complex of Spinach Chloroplasts: Implications for the Pathway of Cyclic Electron Transport
复制标题
铁氧还蛋白：NADP 氧化还原酶，是菠菜叶绿体细胞色素 b6f 复合物的亚基：对循环电子传输途径的影响
DOI：
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发表时间：
2001
期刊：
影响因子：
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通讯作者：
W. Cramer
中科院分区：
文献类型：
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作者：
Huamin Zhang;J. Whitelegge;W. Cramer
作为改善细胞色素b6f复合物的3D晶体质量的努力的一部分，从菠菜类囊体分离的复合物的表征（Huang et al.，1994）和嗜热蓝细菌M. laminosus（Huang等人，（1999年）已延期。在菠菜类囊体的b6f复合物中发现了除细胞色素f和b6以及Rieske ISP外的第四个氧化还原活性亚基，即铁氧还蛋白：NADP氧化还原酶（FNR），但在M.通过相同的方案纯化的层状菌。与纯化的b6f复合物化学计量结合的FNR具有酶活性，这意味着b6f复合物在铁氧化还原非依赖性“循环”电子传递途径中的作用（Zhang et al. 2001年出版）。
Introduction As part of an effort to improve the quality of 3D crystals of the cyochrome b6f complex, the characterization of the complex isolated from spinach thylakoids (Huang et al., 1994) and the thermophilic cyanobacterium M. laminosus (Huang et al., 1999) has been extended. A fourth redox-active subunit in addition to cytochromes f and b6 and the Rieske ISP, ferredoxin:NADP oxidoreductase (FNR), was found in the b6f complex from spinach thylakoids, but not in that from M. laminosus purified by the same protocol. The FNR bound stoichiometrically to the purified b6f complex is enzymatically active, implying a role of the b6f complex in a ferredoxindependent “cyclic” electron transport pathway (Zhang et al., 2001, submitted for publication).