Strain Transfer in Ventricular Cardiomyocytes to Their Transverse Tubular System Revealed by Scanning Confocal Microscopy
Strain Transfer in Ventricular Cardiomyocytes to Their Transverse Tubular System Revealed by Scanning Confocal Microscopy
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DOI：
10.1016/j.bpj.2011.03.046
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发表时间：
2011-05-18
影响因子：
3.4
通讯作者：
Sachse, Frank B.
中科院分区：
文献类型：
--
作者：
McNary, Thomas G.;Bridge, John H. B.;Sachse, Frank B.
横管系统是哺乳动物心室肌细胞的主要信号传递场所，包括电信号传递和兴奋收缩偶联。它由膜内陷组成，膜内陷被各种蛋白质修饰，包括机械敏感离子通道。在这里，我们研究了t系统的机械调制。应用荧光标记、三维扫描共聚焦显微镜和数字图像分析等方法，对不同应变下的离体心室肌细胞进行了研究。我们证明，在细胞水平上的应变被传递到t系统，减少小管的长度和体积，并改变其横截面形状。我们的数据表明，细胞应变低至5%的影响横小管的形状，这具有重要的意义，在他们中发现的机械敏感离子通道的功能。此外，我们的研究支持了先前的假设，即应变可以引起t系统和细胞外空间之间的液体交换。
The transverse tubular system (t-system) is a major site for signaling in mammalian ventricular cardiomyocytes including electrical signaling and excitation-contraction coupling. It consists of membrane invaginations, which are decorated with various proteins including mechanosensitive ion channels. Here, we investigated mechanical modulation of the t-system. By applying fluorescent markers, three-dimensional scanning confocal microscopy, and methods of digital image analysis, we studied isolated ventricular cardiomyocytes under different strains. We demonstrate that strain at the cellular level is transmitted to the t-system, reducing the length and volume of tubules and altering their cross-sectional shape. Our data suggest that a cellular strain of as little as 5% affects the shape of transverse tubules, which has important implications for the function of mechanosensitive ion channels found in them. Furthermore, our study supports a prior hypothesis that strain can cause fluid exchange between the t-system and extracellular space.