Unphysiologically high magnesium concentrations support chondrocyte proliferation and redifferentiation
Unphysiologically high magnesium concentrations support chondrocyte proliferation and redifferentiation
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DOI：
10.1089/ten.2006.12.3545
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发表时间：
2006-12-01
影响因子：
--
通讯作者：
Willumeit, Regine
中科院分区：
文献类型：
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作者：
Feyerabend, Frank;Witte, Frank;Willumeit, Regine
采用三期组织工程模型研究了非生理性高细胞外镁浓度对硫酸镁诱导的软骨细胞的影响。实验表明，在基因和蛋白表达水平上，软骨细胞增殖和再分化得到增强。在软骨形成过程中发现了负面影响，其中观察到抑制细胞外基质的形成。此外，除了直接影响软骨细胞代谢外，镁浓度升高还通过在软骨形成过程中的连续影响影响生长因子的有效性。所有观察结果均与剂量相关。本研究结果表明，镁可能是软骨组织工程的有用工具。
The effect of unphysiologically high extracellular magnesium concentrations on chondrocytes, induced by the supplementation of magnesium sulfate, was studied using a 3-phase tissue engineering model. The experiments showed that chondrocyte proliferation and redifferentiation, on the gene and protein expression level, are enhanced. A negative influence was found during chondrogenesis where an inhibition of extracellular matrix formation was observed. In addition, a direct impact on chondrocyte metabolism, elevated magnesium concentrations also affected growth factor effectiveness by consecutive influences during chondrogenesis. All observations were dosage dependent. The results of this study indicate that magnesium may be a useful tool for cartilage tissue engineering.