Capillary electrophoresis of some tetracycline antibiotics
Capillary electrophoresis of some tetracycline antibiotics
复制标题
某些四环素类抗生素的毛细管电泳
DOI：
10.1016/0021-9673(94)85043-7
复制
发表时间：
1994
影响因子：
4.1
通讯作者：
C. Peteghem
中科院分区：
文献类型：
--
作者：
S. Croubels;W. Baeyens;C. Dewaele;C. Peteghem
Data on the separation of tetracycline antibiotics by capillary electrophoresis are rather limited and have not been reported for micellar electrokinetic capillary chromatographic separation (MECC). In the present study, the separation of tetracycline, oxytetracycline and chlortetracycline by capillary zone electrophoresis and MECC was investigated. Adding non-ionic surfactants such as Triton X-100 to a 0.2Mphosphate migration buffer of pH 2.2 greatly improved separation. The use of mixed micelles enlarged the variety of the micellar phases,e.g.a combination of Tween 20 and Tween 80 provided a similar separation pattern. The addition of β-cyclodextrin to a Triton X-100 and Brij-35 surfactant combination did not result in an improved separation. A Triton X-100 and Brij-35 combination could separate tetracycline and its degradation products 4-epitetracycline (ETC), anhydrotetracycline and 4-epianhydrotetracycline. This enabled us to identify ETC in a commercial tetracycline sample.
