Fragments of the simian virus 40 transforming gene facilitate transformation of rat embryo cells.
Fragments of the simian virus 40 transforming gene facilitate transformation of rat embryo cells.
复制标题
猿猴病毒 40 转化基因的片段有利于大鼠胚胎细胞的转化。
DOI：
10.1073/pnas.79.17.5189
复制
发表时间：
1982
影响因子：
11.1
通讯作者：
Shenk,T
中科院分区：
文献类型：
--
作者：
Colby,WW;Shenk,T
猴病毒40（SV 40）基因组中仅编码大肿瘤抗原片段的片段可以促进大鼠次级胚胎细胞的永生化。永生化细胞的表型范围从接近“正常”到完全转化。所有的细胞系都含有SV 40序列，并表达大肿瘤抗原的不稳定的NH 2-末端片段。SV 40小肿瘤抗原似乎不是永生化或转化所必需的。
Segments of the simian virus 40 (SV40) genome that encode only fragments of large tumor antigen can facilitate immortalization of secondary rat embryo cells. The phenotypes of the immortalized cells range from nearly "normal" to fully transformed. All of the cell lines contain SV40 sequences and express unstable NH2-terminal fragments of large tumor antigen. SV40 small tumor antigen does not appear to be essential for either immortalization or transformation.