AMIGO is expressed in multiple brain cell types and may regulate dendritic growth and neuronal survival
AMIGO is expressed in multiple brain cell types and may regulate dendritic growth and neuronal survival
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DOI：
10.1002/jcp.22958
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发表时间：
2012-05-01
影响因子：
5.6
通讯作者：
Tang, Bor Luen
中科院分区：
文献类型：
--
作者：
Chen, Yanan;Hor, Hong Huan;Tang, Bor Luen
两性蛋白诱导基因和 ORF (AMIGO) 是一种脑富集的跨膜免疫球蛋白 (Ig) 超家族蛋白，具有六个富含亮氨酸的细胞外重复序列 (LRR) 和单个免疫球蛋白样 (Ig) 结构域。我们在此报告，AMIGO 是一种在中枢神经系统 (CNS) 中广泛表达的糖基化蛋白，可以在神经元、星形胶质细胞和少突胶质细胞中找到。在形态成熟的原代神经元中，内源性 AMIGO 和转染的全长 AMIGO (AMIGO-FL) 主要是树突状，而删除 LRR 结构域的 AMIGO (AMIGO-Ig) 主要是轴突。与 AMIGO 的树突定位一致，siRNA 介导的 AMIGO 沉默导致培养物中皮层神经元的树突生长减少。与载体对照相比，稳定过表达 AMIGO 的 SH-SY5Y 细胞对十字孢菌素和 H2O2 诱导的细胞凋亡具有更强的抵抗力。因此，AMIGO 可能在发育过程中的树突生长中发挥重要作用，并且可以调节发育中和成年神经元的存活。 J.细胞。生理学。 227：2217-2229，2012 年。(C) 2011 Wiley 期刊公司。
Amphoterin-induced gene and ORF (AMIGO) is a brain-enriched transmembrane immunoglobulin (Ig) superfamily protein with six extracellular leucine-rich repeats (LRR) and a single immunoglobulin-like (Ig) domain. We report here that AMIGO is a glycosylated protein widely expressed in the central nervous system (CNS), and can be found in neurons, astrocytes as well as oligodendrocytes. In morphologically mature primary neurons, endogenous AMIGO, and transfected full length AMIGO (AMIGO-FL) are largely dendritic, while AMIGO with its LRR domain deleted (AMIGO-Ig) is predominantly axonal. In line with AMIGO's dendritic localization, siRNA-mediated silencing of AMIGO resulted in reduced dendritic growth of cortical neurons in culture. SH-SY5Y cells stably over-expressing AMIGO are more resistant to apoptosis induced by staurosporine and H2O2 compared to vector controls. AMIGO therefore likely plays important roles in dendritic outgrowth during development, and could modulate the survival of developing and adult neurons. J. Cell. Physiol. 227: 2217-2229, 2012. (C) 2011 Wiley Periodicals, Inc.