Electrophysiological Approaches for the Study of Ion Channel Function.
Electrophysiological Approaches for the Study of Ion Channel Function.
复制标题
DOI：
10.1007/978-1-0716-1394-8_4
复制
发表时间：
2021
期刊：
影响因子：
--
通讯作者：
McCarty NA
中科院分区：
文献类型：
--
作者：
Cui G;Cottrill KA;McCarty NA
离子通道在细胞生理学中起着至关重要的作用，是临床相关药物的主要靶点。由于它们携带离子电流，离子通道的功能和药理学可以使用电生理学方法进行研究，其分辨率范围从单分子到数百万分子。本章描述了一种用于研究遗传性疾病中有缺陷的典型离子通道的电生理方法，这是目前临床上使用的所谓高效调节剂疗法的目标：囊性纤维化跨膜传导调节剂（CFTR）。提供了研究CFTR异种表达、CFTR在气道上皮细胞原位表达、纯化或部分纯化的CFTR蛋白重组成平面脂质双层的方案。
Ion channels play crucial roles in cell physiology, and are a major class of targets for clinically relevant pharmaceuticals. Because they carry ionic current, the function and pharmacology of ion channels can be studied using electrophysiological approaches that range in resolution from the single molecule to many millions of molecules. This chapter describes electrophysiological approaches for the study of one representative ion channel that is defective in a genetic disease, and that is the target of so-called highly effective modulator therapies now used in the clinic: the cystic fibrosis transmembrane conductance regulator (CFTR). Protocols are provided for studying CFTR expressed heterologously, for CFTR expressed in situ in airway epithelial cells, and for purified or partially purified CFTR protein reconstituted into planar lipid bilayers.