Decrease in mast cells in oral squamous cell carcinoma:: Possible failure in the migration of these cells
Decrease in mast cells in oral squamous cell carcinoma:: Possible failure in the migration of these cells
复制标题
DOI：
10.1016/j.oraloncology.2006.05.004
复制
发表时间：
2007-05-01
期刊：
影响因子：
4.8
通讯作者：
Batista, Aline Carvalho
中科院分区：
文献类型：
--
作者：
Oliveira-Neto, Helenisa Helena;Leite, Angelica Ferreira;Batista, Aline Carvalho
人们逐渐认识到基质中有多种细胞类型。微环境参与肿瘤发生。在这种情况下，肥大细胞（MC）表现出多种作用，可能有助于防御肿瘤或肿瘤进展。因此，本研究的目的是评估 OSCC（口腔鳞状细胞癌）和癌前口腔角化过度（白斑）中 MC 的密度和迁移，以及它们与临床和显微镜参数的关系。采用免疫组化方法分析38例口腔鳞状细胞癌、26例白斑病和12例临床健康口腔粘膜（对照）中类胰蛋白酶和c-kit的表达。与对照组相比，OSCC（P = 0.0003）和白斑（P = 0.03）中类胰蛋白酶（+）细胞数量减少。在白斑和 OSCC 中观察到类似数量的类胰蛋白酶 (+) 细胞 (P = 0.31)。与对照相比，口腔鳞癌和白斑中 c-kit(+) MC 的密度也显着升高，导致与白斑 (59%) 和对照 (63%) 相比，口腔鳞癌 (19%) 中 c-kit(+)/类胰蛋白酶 (+) 关系降低。未观察到 MC 人群与 OSCC 的临床和显微镜特征之间存在相关性。我们的研究结果表明，恶变前和恶性口腔病变中 MC 数量的减少可能与这些细胞的迁移失败有关，可能反映了肿瘤发生和进展过程中微环境的重要改变。 (c) 2006 Elsevier Ltd. 保留所有权利。
It is becoming accepted that multiple cell types in stromal. microenvironment are involved in tumorigenesis. In this setting, mast cells (MC) display a diversity of roles that may contribute to the defense against tumors or tumor progression. Thus, the aim of this study was to evaluate density and migration of MCs in OSCC (oral squamous cell carcinoma) and premalignant oral hyperkeratosis (leukoplakia) as well as their relationship with clinical and microscopic parameters. The tryptase and c-kit expression was analyzed in 38 cases of OSCC, 26 cases of leukoplakia, and 12 cases of clinically healthy oral mucosa (control) by means of immunohistochemistry. The tryptase(+) cell numbers were decreased in OSCC (P = 0.0003) and leukoplakia (P = 0.03) compared with control. Similar numbers of tryptase(+) cells were observed in leukoplakia and OSCC (P = 0.31). The density of c-kit(+) MCs was also significantly tower in OSCC and leukoplakia in relation to control resulting in a reduced c-kit(+)/tryptase(+) relationship in OSCC (19%) in comparison with leukoplakia (59%) and control (63%). No correlation was observed between MC poputations with clinical and microscopic characteristics of OSCC. Our findings suggest that the decrease in MC numbers in pre-malignant and malignant oral lesions may be related to the migration failure of these cells, possibly reflecting an important modification in the microenvironment during tumor initiation and progression. (c) 2006 Elsevier Ltd. All rights reserved.