Biotransformation of two-line silica-ferrihydrite by a dissimilatory Fe(III)-reducing bacterium: Formation of carbonate green rust in the presence of phosphate
Biotransformation of two-line silica-ferrihydrite by a dissimilatory Fe(III)-reducing bacterium: Formation of carbonate green rust in the presence of phosphate
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DOI：
10.1016/j.gca.2003.12.024
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发表时间：
2004-07-01
影响因子：
5
通讯作者：
Kennedy, DW
中科院分区：
文献类型：
--
作者：
Kukkadapu, RK;Zachara, JM;Kennedy, DW
以乳酸为电子供体，研究了希瓦氏菌CN32在pH近似于7的1,4-哌嗪二烷基磺酸缓冲介质中对两种Si-铁水合物（1和5摩尔% Si）的还原性生物转化。蒽醌-2,6-二磺酸盐是一种电子穿梭体，存在于介质中。在含铁50 mmol/L的培养基中，分别进行无PO43- (P)和添加PO43- (P) （1 ~ 20 mmol/L）的实验。我们的目的是确定SiO4- (Si)和P在缺氧条件下对水合铁的生物可还原性和生物矿化的综合影响。测定了铁的还原率随时间的变化，用粉末x射线衍射和穆斯堡尔光谱对固体进行了表征，并对水溶液中的Si、P、Cl和无机碳进行了分析。无论Si和P含量如何，这两种铁水合体都能迅速还原。Si浓度对还原速率和矿化产物没有影响。碳酸盐绿锈GR(CO32-)((Fe(6-x)FexIII)-Fe- ii (OH)(12)](x+)(CO32-)（0.5）。yH(2)O)和活石[Fe-3(PO4)(2)]。当P存在时形成8H(2)O]。以GR（CO32-）为主的矿物产物
The reductive biotransformation of two Si-ferrihydrite coprecipitates (1 and 5 mole % Si) by Shewanella putrefaciens, strain CN32, was investigated in 1,4-piperazinediethanesulfonic acid-buffered media (pH similar to7) with lactate as the electron donor. Anthraquinone-2,6-disulfonate, an electron shuttle, was present in the media. Experiments were performed without and with PO43- (P) (1 to 20 mmol/L) in media containing 50 mmol/L Fe. Our objectives were to define the combined effects of SiO4- (Si) and P on the bioreducibility and biominer4 alization of ferrihydrites under anoxic conditions. Iron reduction was measured as a function of time, solids were characterized by powder X-ray diffraction and Mossbauer spectroscopy, and aqueous solutions were analyzed for Si, P, Cl- and inorganic carbon. Both of the ferrihydrites were rapidly reduced regardless of the Si and P content. Si concentration had no effect on the reduction rate or mineralization products. Magnetite was formed in the absence of P whereas carbonate green rust GR(CO32-)((Fe(6-x)FexIII)-Fe-II(OH)(12)](x+)(CO32-)(0.5) . yH(2)O) and vivianite [Fe-3(PO4)(2) . 8H(2)O] were formed when P was present. GR(CO32-) dominated as a mineral product in samples with