Biosynthesis of silver nanoparticle and its application in cell wall disruption to release carbohydrate and lipid from C. vulgaris for biofuel production.
Biosynthesis of silver nanoparticle and its application in cell wall disruption to release carbohydrate and lipid from C. vulgaris for biofuel production.
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DOI：
10.1016/j.btre.2016.07.001
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发表时间：
2016-09
期刊：
影响因子：
--
通讯作者：
Mani, Vijay
中科院分区：
文献类型：
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作者：
Abdul Razack, Sirajunnisa;Duraiarasan, Surendhiran;Mani, Vijay
从土壤分离的枯草芽孢杆菌中成功地合成了AgNP。所合成的AgNPs经UV-Spec、FTIR、TEM和X射线衍射仪确证。这些AgNPs被成功地用微藻处理，以破坏其细胞壁，释放脂肪和碳水化合物。乳酸脱氢酶(LDH)和扫描电子显微镜(SEM)证实细胞壁损伤。微藻是一种羽翼未丰的原料，可生产生产第三代生物燃料的原材料。各种破壁技术和常规破壁技术都有助于提取脂肪和碳水化合物，但这些缺点促使生物技术工作者探索更快、更经济地裂解细胞壁的新工艺。银纳米颗粒具有打破微藻细胞壁、有效释放生物分子的能力。利用一株新型细菌枯草芽孢杆菌实现了银纳米颗粒的绿色合成。分析了纳米银的性质及其对微藻细胞壁裂解的影响。乳酸脱氢酶检测证实细胞壁损伤，扫描电子显微镜观察细胞壁损伤。这项关于直接使用纳米颗粒裂解细胞壁的第一项研究工作可能会比传统方法更具优势，并且是一种更环保、更具成本效益和不费力地生产生物柴油的方法。
The AgNP was successfully synthesised from soil isolated bacterium Bacillus subtilis. The synthesised AgNPs were confirmed by UV-Spec, FTIR, TEM and XRD. The AgNPs were successfully treated microalga to disrupt its cell wall to release lipids and carbohydrates. The cell wall damages evidenced by LDH assay and SEM. Microalgae are the fledging feedstocks yielding raw materials for the production of third generation biofuel. Assorted and conventional cell wall disruption techniques were helpful in extracting lipids and carbohydrates, nevertheless the disadvantages have led the biotechnologists to explore new process to lyse cell wall in a faster and an economical manner. Silver nanoparticles have the ability to break the cell wall of microalgae and release biomolecules effectively. Green synthesis of silver nanoparticles was performed using a novel bacterial isolate of Bacillus subtilis. Characterisation of nanosilver and its effect on cell wall lysis of microalgae were extensively analysed. Cell wall damage was confirmed by lactate dehydrogenase assay and visually by SEM analysis. This first piece of research work on direct use of nanoparticles for cell wall lysis would potentially be advantageous over its conventional approaches and a greener, cost effective and non laborious method for the production of biodiesel.