Monitoring the Inflammatory Response to Infection through the Integration of MALDI IMS and MRI
Monitoring the Inflammatory Response to Infection through the Integration of MALDI IMS and MRI
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DOI：
10.1016/j.chom.2012.04.018
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发表时间：
2012-06-14
影响因子：
30.3
通讯作者：
Skaar, Eric P.
中科院分区：
文献类型：
--
作者：
Attia, Ahmed S.;Schroeder, Kaitlin A.;Skaar, Eric P.
全身性细菌感染的特征在于强烈的全机体炎症反应。对感染的免疫反应的分析涉及通常专注于单个器官系统并且缺乏空间信息的技术。此外，单个炎性蛋白的分析需要对感兴趣的蛋白具有特异性的抗体，这限制了可以分析的蛋白质组。在这里，我们描述了应用基质辅助激光解吸/电离成像质谱（MALDI IMS）的小鼠全身感染金黄色葡萄球菌，以确定炎症蛋白质群众，整个感染动物的感染。将磁共振成像（MRI）提供的分辨率与MALDI IMS的灵敏度相结合，提供了关于全身感染期间先天免疫蛋白分布的三维空间分辨信息，允许通过MRI与体内结构信息和软组织对比进行比较。因此，将MALDI IMS与MRI相结合提供了一种系统生物学方法来研究感染期间的炎症。
Systemic bacterial infection is characterized by a robust whole-organism inflammatory response. Analysis of the immune response to infection involves technologies that typically focus on single organ systems and lack spatial information. Additionally, the analysis of individual inflammatory proteins requires antibodies specific to the protein of interest, limiting the panel of proteins that can be analyzed. Herein we describe the application of matrix-assisted laser desorption/ionization imaging mass spectrometry (MALDI IMS) to mice systemically infected with Staphylococcus aureus to identify inflammatory protein masses that respond to infection throughout an entire infected animal. Integrating the resolution afforded by magnetic resonance imaging (MRI) with the sensitivity of MALDI IMS provides three-dimensional spatially resolved information regarding the distribution of innate immune proteins during systemic infection, allowing comparisons to in vivo structural information and soft-tissue contrast via MRI. Thus, integrating MALDI IMS with MRI provides a systems-biology approach to study inflammation during infection.