Manipulation and trapping of sub-micron bioparticles using dielectrophoresis
Manipulation and trapping of sub-micron bioparticles using dielectrophoresis
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DOI：
10.1016/s0165-022x(97)00033-x
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发表时间：
1997-09-25
影响因子：
--
通讯作者：
Milner, JJ
中科院分区：
文献类型：
--
作者：
Green, NG;Morgan, H;Milner, JJ
非均匀交变电场引起可极化粒子的运动，称为介电泳。这种效应取决于介质和粒子的介电性质的相对大小。该技术已被用于操纵生物技术应用的颗粒，包括细胞和微生物的纯化、分级和浓缩。然而，介电泳操纵的颗粒的尺寸下限被认为是约1 μ m，但最近的工作已经证明，否则。用微加工电极结构测量了乳胶珠和一种简单杆状病毒烟草花叶病毒（TMV）的介电电泳运动和性质。测量了悬浮介质的电导率、外加频率和电场强度。结果表明，在适当的条件下，乳胶珠和烟草花叶病毒颗粒可以选择性地吸引到高电场强度位于微电极结构的尖端的区域。选择性捕获和分离生物颗粒的能力在生物技术领域具有许多潜在的应用。(C)1997年Elsevier Science B.V.
A non-uniform alternating electric field induces motion in polarisable particles called dielectrophoresis. The effect is governed by the relative magnitudes of the dielectric properties of the medium and the particles. The technology has been used to manipulate particles for biotechnological applications, including purification, fractionation and concentration of cells and microorganisms. However, the lower size limit for the dielectrophoretic manipulation of particles was believed to be about 1 mu m, but recent work has proved otherwise. The dielectrophoretic movement and properties of latex beads and a simple rod-shaped virus, tobacco mosaic virus (TMV), have been measured using microfabricated electrode structures. Measurements have been made over a range of suspending medium conductivities, applied frequencies and electric field strengths. It is shown that under appropriate conditions both latex beads and tobacco mosaic virus particles can be selectively attracted to regions of high electric field strength located at the tips of microfabricated electrode structures. The ability to selectively trap and separate bio-particles has many potential applications in the area of biotechnology. (C) 1997 Elsevier Science B.V.