Expression and characterization of disulfide bond use of oligomerized A2-pancortins: Extracellular matrix constituents in the developing brain
Expression and characterization of disulfide bond use of oligomerized A2-pancortins: Extracellular matrix constituents in the developing brain
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DOI：
10.1016/j.neuroscience.2005.03.009
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发表时间：
2005-01-01
期刊：
影响因子：
3.3
通讯作者：
Sato, M
中科院分区：
文献类型：
--
作者：
Ando, K;Nagano, T;Sato, M
细胞外基质的区域特异性特征对于脑中的多种功能至关重要。泛皮质素/神经元特异性嗅觉调节素相关糖蛋白是细胞外基质的组分。它们包括四种选择性剪接变体，Pancortin-1至-4，它们共有一个共同的部分，即B部分，在其结构的中间，具有两对选择性剪接的5'区A1和A2，以及3'区C1和C2。在这里，我们证明，在小鼠中，Pancortin-3（A2-B-C1）和Pancortin-4（A2-B-C2），我们将其归类为A2-Pancortin，在大脑发育的早期以区域特异性方式转录，并在体内非常稳定地表达。它们被N-糖基化并分泌。此外，我们研究了他们的个体发育中的丘脑使用抗血清的共同B区的表达谱，因为他们的mRNA的瞬时表达是显着的。在发育中的丘脑中，它们以寡聚化的形式持续很长时间，即使在它们的mRNA转录降低到无法检测的水平之后。进一步的分析表明，位于共同B部分的半胱氨酸残基对于A2-Pancortin的同源和异源寡聚体形成是重要的。当我们用丝氨酸残基取代共同B部分中的半胱氨酸残基45和47时，A2-Pancortin的寡聚化受到高度干扰。(c)2005由Elsevier Ltd代表IBRO出版。
The region-specific characteristics of the extracellular matrix are crucial for diverse functions in the brain. Pancortins/neuron-specific olfactomedin-related glycoproteins are components of the extracellular matrix. They comprise four alternatively spliced variants, Pancortin-1 to -4, which share a common portion, the B part, in the middle of their structure, have two pairs of alternatively spliced 5' regions, A1 and A2, and 3' regions, C1 and C2. Here we demonstrate that in mice, Pancortin-3 (A2-B-C1) and Pancortin-4 (A2-B-C2), which we have grouped together the A2-Pancortins, were transcribed early during the development of the brain in a region specific manner and were expressed very stably in vivo. They are N-glycosylated and secreted. Furthermore, we examined their ontogenetical expression profiles in the developing thalamus using antiserum against the common B region, since transient expressions of their mRNAs were notable there. In the developing thalami, they lasted long in oligomerized form even after the transcription of their mRNAs decreased to an undetectable level. Further analyses revealed that cysteine residues that are located in the common B part are important for homo-and hetero-oligomer formation of A2-Pancortins. When we substituted cysteine residues 45 and 47 with serine residues in that common B part, oligomerization of the A2-Pancortins was highly disturbed. (c) 2005 Published by Elsevier Ltd on behalf of IBRO.