Enterococcus faecalis-mediated biomineralized biofilm formation on root canal dentine in vitro
Enterococcus faecalis-mediated biomineralized biofilm formation on root canal dentine in vitro
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DOI：
10.1002/jbm.a.30622
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发表时间：
2006-05-01
影响因子：
4.9
通讯作者：
Kumar, R
中科院分区：
文献类型：
--
作者：
Kishen, A;George, S;Kumar, R
粪肠球菌是根管治疗失败的牙齿中最主要的细菌，并且被发现在根管治疗牙齿的根管中普遍存在的恶劣条件下存活。本研究旨在探讨E.粪便和根管牙本质基质。为此，制备牙齿标本并分为两组。第1组牙体标本与E.粪的时间为2-，4-，和6周的时间间隔，并使用X-射线衍射和傅里叶变换红外（FTIR）光谱测定的生物膜的化学组成。第2组牙体标本与E.粪的一段时间的6周，并使用扫描电子显微镜（SEM），光学显微镜，和激光共聚焦扫描显微镜检查的生物膜的地形和超微结构。用扫描电镜和傅立叶变换红外光谱对细菌作用于牙本质表面形成的沉积物进行了观察。这些实验突出了粪肠球菌与根管牙本质相互作用的不同阶段。此外，还观察到细菌1诱导的磷灰石在成熟生物膜上的再沉淀。E.粪菌在根管牙本质上形成这种钙化生物膜可能是导致其在牙髓治疗后持续存在的一个因素。(c)2006 Wiley Periodicals，Inc.
Enterococcus faecalis is the most predominant bacteria in teeth with failed root canal therapy and is found to survive harsh conditions prevailing in the root canals of endodontically treated teeth. This study aims to investigate the interaction between E. faecalis and root canal dentine substrate. Towards this end, tooth specimens were prepared and divided into two groups. The tooth specimens in group 1 were incubated with E. faecalis for periods of 2-, 4-, and 6-week intervals and the chemical composition of the biofilm was determined using X-ray diffraction and Fourier transform infrared (FTIR) spectroscopy. The tooth specimens in group 2 were incubated with E. faecalis for a period of 6 weeks and the topography and ultrastructure of the biofilm were examined using scanning electron microscopy (SEM), light microscopy, and laser confocal scanning microscopy. The sediments formed from the bacterial interaction on the dentine (in group 1) were also examined by SEM and FTIR. These experiments highlighted different stages in the interaction of E.faecalis with root canal dentine. Further, a bacteria 1-induced apatite reprecipitation on mature biofilm was also observed. This ability of E. faecalis to form such calcified biofilm on root canal dentine may be a factor that contributes to their persistence after endociontic treatment. (c) 2006 Wiley Periodicals, Inc.