Anti-proliferative effect of horehound leaf and wild cherry bark extracts on human colorectal cancer cells.
Anti-proliferative effect of horehound leaf and wild cherry bark extracts on human colorectal cancer cells.
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DOI：
10.3892/or.15.1.275
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发表时间：
2006
期刊：
影响因子：
4.2
通讯作者：
K. Yamaguchi;J. Liggett;Nam-Cheol Kim;S. Baek
中科院分区：
文献类型：
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作者：
K. Yamaguchi;J. Liggett;Nam-Cheol Kim;S. Baek
Marubium vulgare（苦苣）和Prunus sertina（野樱桃）传统上被用于治疗与炎症相关的症状，如感冒、发烧和喉咙痛。在本报告中，我们发现抗炎的苦苣苔叶和野生樱桃树皮提取物对人类结直肠癌细胞具有抗增殖活性。苦根和野樱桃提取物均能抑制细胞生长和诱导细胞凋亡。我们发现苦根提取物通过反激活促凋亡非甾体抗炎药激活基因（NAG-1）来上调促凋亡非甾体抗炎药激活基因（NAG-1）。相比之下，野生樱桃提取物在HCT-116和SW480细胞系中降低了cyclin D1的表达，增加了NAG-1的表达。通过TOP/FOP报告构建评估，野樱桃提取物可抑制β -catenin/T细胞因子转录，表明野樱桃提取物抑制β -catenin信号通路可导致cyclin D1表达降低。我们的数据表明，这些提取物抑制细胞生长和诱导细胞凋亡的机制包括增强NAG-1表达和/或下调β -连环蛋白信号传导，随后降低人类结直肠癌细胞中cyclin D1的表达。这些发现可能为传统抗炎产品作为癌症化学预防剂提供机制。
Marubium vulgare (horehound) and Prunus serotina (wild cherry) have been traditionally used for the treatment of inflammatory-related symptoms such as cold, fever, and sore throat. In this report, we show that extracts of anti-inflammatory horehound leaves and wild cherry bark exhibit anti-proliferative activity in human colorectal cancer cells. Both horehound and wild cherry extracts cause suppression of cell growth as well as induction of apoptosis. We found that horehound extract up-regulates pro-apoptotic non-steroidal anti-inflammatory drug-activated gene (NAG-1) through transactivation of the NAG-1 promoter. In contrast, wild cherry extract decreased cyclin D1 expression and increased NAG-1 expression in HCT-116 and SW480 cell lines. Treatment with wild cherry extract resulted in the suppression of beta-catenin/T cell factor transcription, as assessed by TOP/FOP reporter constructs, suggesting that suppressed beta-catenin signaling by wild cherry extract leads to the reduction of cyclin D1 expression. Our data suggest the mechanisms by which these extracts suppress cell growth and induce apoptosis involve enhanced NAG-1 expression and/or down-regulation of beta-catenin signaling, followed by reduced cyclin D1 expression in human colorectal cancer cells. These findings may provide mechanisms for traditional anti-inflammatory products as cancer chemopreventive agents.