Optical imaging of cell mass and growth dynamics
Optical imaging of cell mass and growth dynamics
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DOI：
10.1152/ajpcell.00121.2008
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发表时间：
2008-08-01
影响因子：
5.5
通讯作者：
Badizadegan, Kamran
中科院分区：
文献类型：
--
作者：
Popescu, Gabriel;Park, YoungKeun;Badizadegan, Kamran
利用新的干涉定量位相显微镜方法，我们证明了与活细胞相关的光学相的表面积分与细胞水分含量是不变的。因此，我们提供了一种完全非侵入性的方法来测量活细胞的非水含量或“干质量”。鉴于干涉显微镜的极高稳定性和该方法对细胞干质量变化的飞秒图敏感性，这项新技术不仅是量化细胞生长的理想方法，而且还在时间尺度上揭示了活细胞数十年的空间分辨细胞和亚细胞动力学。具体地说，我们给出了单个细胞质量的定量直方图，描述了高糖在系膜细胞模型中的肥大效应。此外，我们还发现，在一个长时间观察的上皮细胞模型中，平均平方位移数据揭示了不同特征长度和时间尺度上的细胞和亚细胞动力学的特定信息。总体而言，这项研究表明，干涉计量定量位相显微镜代表了一种非侵入性的光学分析方法，用于监测细胞生长、表征细胞运动和研究活细胞的亚细胞运动。
Using novel interferometric quantitative phase microscopy methods, we demonstrate that the surface integral of the optical phase associated with live cells is invariant to cell water content. Thus, we provide an entirely noninvasive method to measure the nonaqueous content or " dry mass" of living cells. Given the extremely high stability of the interferometric microscope and the femtogram sensitivity of the method to changes in cellular dry mass, this new technique is not only ideal for quantifying cell growth but also reveals spatially resolved cellular and subcellular dynamics of living cells over many decades in a temporal scale. Specifically, we present quantitative histograms of individual cell mass characterizing the hypertrophic effect of high glucose in a mesangial cell model. In addition, we show that in an epithelial cell model observed for long periods of time, the mean squared displacement data reveal specific information about cellular and subcellular dynamics at various characteristic length and time scales. Overall, this study shows that interferometeric quantitative phase microscopy represents a noninvasive optical assay for monitoring cell growth, characterizing cellular motility, and investigating the subcellular motions of living cells.