A decrease in 1H nuclear magnetic resonance spectroscopically determined citrate in human seminal fluid accompanies the development of prostate adenocarcinoma
A decrease in 1H nuclear magnetic resonance spectroscopically determined citrate in human seminal fluid accompanies the development of prostate adenocarcinoma
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DOI：
10.1097/01.ju.0000148949.72314.d7
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发表时间：
2005-02-01
影响因子：
6.6
通讯作者：
Sillerud, LO
中科院分区：
文献类型：
--
作者：
Averna, TA;Kline, EE;Sillerud, LO
目的：由于人类前列腺液占精浆体积的近50%，且前列腺液中含有独特的前列腺代谢物，如柠檬酸盐，在肿瘤发生过程中会发生显著变化，因此我们研究了未经处理的人精浆的高分辨率核磁共振氢谱，作为前列腺癌的快速、非侵入性诊断工具。材料和方法：来自对照组和荷瘤受试者的精液和前列腺按摩样品在水抑制核磁共振分析之前被冷冻和解冻。我们发现冷冻对精液核磁共振谱没有显著影响。结果：3名前列腺特异性抗原(PSA)<1 ng/ml的对照受试者精浆中的柠檬酸共振谱分辨率较好，其浓度为97~178 mM。一名47岁男子的精液。良性前列腺增生症患者的PSA为5.5 ng/ml，含有156 mM的柠檬酸盐。相比之下，2名前列腺癌患者的精浆：包括一名46岁的男性，格里森8级，PSA 45.2 ng/ml。，6级，PSA13.0 ng/ml，核磁共振信号显示柠檬酸盐浓度分别为28和24 mM。23岁正常人前列腺液中柠檬酸根含量为483 mm，而56岁格里森4级癌患者前列腺液中柠檬酸根含量仅为1.35 nm。结论：本研究首次将精液高分辨核磁共振用于前列腺癌诊断。鉴于腺癌对前列腺癌代谢的已知影响，该研究表明，高分辨率H-1核磁共振可用于测量精液中的柠檬酸盐：潜在地提供了一种新的、快速的、非侵入性的筛查方法。
Purpose: Because human prostatic fluid contributes almost 50% of the volume of seminal plasma and this fluid contains unique prostatic metabolites such as citrate, which are markedly altered during tumorigenesis, we investigated high resolution H-1 nuclear magnetic resonance (NMR) spectroscopy of unprocessed human seminal plasma as a rapid, noninvasive diagnostic tool for prostate adenocarcinoma.Materials and Methods: Semen and prostatic massage samples from control and tumor bearing subjects were stored frozen at -20C and thawed prior to water suppressed NMR analysis. We found that freezing produced no significant alterations in the semen NMR spectra. Quantitative NMR spectroscopy was performed by first calibrating the water suppression data acquisition sequence with a series of standard samples containing known amounts of citrate within the physiological range.Results: Well resolved citrate resonances from the seminal plasma of 3 control subjects with prostate specific antigen (PSA) less than 1 ng/ml were integrated to give concentrations of 97 to 178 mM. Semen from a 47-year-old. man with benign prostatic hyperplasia and a PSA of 5.5 ng/ml contained 156 mM citrate. In contrast, seminal plasma from 2 patients with prostate cancer: including a 46-year-old man with Gleason grade 8 and PSA 45.2 ng/ml,. and a 64-year-old man with grade 6 and PSA 13.0 ng/ml, revealed citrate NMR signals corresponding to a concentration of only 28 and 24 mM, respectively. Spectra from prostatic massage fluid from a normal 23-year-old volunteer showed a citrate of 483 mM, while massage fluid from a 56-year-old patient with Gleason grade 4 cancer showed a citrate of only 1.35 nM.Conclusions: To our knowledge this study is the first to use high resolution NMR of semen to diagnose prostate cancer. Given the known effects of adenocarcinoma on prostate metabolism, the study indicates that high resolution H-1 NMR can be used to measure citrate in seminal fluid: potentially providing a new, rapid, noninvasive screening method.