Intraviral interactome of Chikungunya virus reveals the homo-oligomerization and palmitoylation of structural protein TF
Intraviral interactome of Chikungunya virus reveals the homo-oligomerization and palmitoylation of structural protein TF
复制标题
基孔肯雅病毒的病毒内相互作用组揭示了结构蛋白 TF 的同源寡聚化和棕榈酰化
DOI：
10.1016/j.bbrc.2019.04.098
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发表时间：
2019
影响因子：
3.1
通讯作者：
Leiliang Zhang
中科院分区：
文献类型：
--
作者：
Han Yin;Peiqi Yin;Hongjian Zhao;Na Zhang;Xia Jian;Siqi Song;Shan Gao;Leiliang Zhang
基孔肯雅病毒(CHIKV)是一种重新出现的蚊子传播的RNA病毒，会导致关节和肌肉疼痛。虽然CHIKV非结构蛋白之间的蛋白质-蛋白质相互作用(PPI)已被广泛建立，但CHIKV病毒内完整的相互作用组仍有待阐明。在本研究中，我们用免疫沉淀法检测了所有可能的CHIKV病毒内PPI，并构建了CHIKV病毒内相互作用体。我们报道了19个新的PPI，其中包括Tf的均相齐聚。二硫键促进了CHIKV Tf蛋白的齐聚。棕榈酰化抑制剂2-BP减少了TF的棕榈酰化，增加了TF的寡聚化。在Tf中，Cys33、Cys35、Cys41和Cys43的四重突变体阻断了其棕榈酰化，减少了寡聚。此外，我们以棕榈酰化依赖的方式确定了Tf与nsP1和NSP3的关联。CHIKV病毒内相互作用体的构建为进一步研究CHIKV蛋白的功能奠定了基础。
Chikungunya virus (CHIKV) is a re-emerging mosquito-transmitted RNA virus causing joint and muscle pain. Although the protein-protein interactions (PPIs) between nonstructural proteins of CHIKV have been extensively established, the complete CHIKV intraviral interactome remains to be elucidated. In this study, we examined all possible CHIKV intraviral PPIs by immunoprecipitation and constructed the intraviral interactome of CHIKV. We reported 19 novel PPIs including the homo-oligomerization of TF. Disulfide bonds promoted the oligomerization of CHIKV TF protein. 2-BP, a palmitoylation inhibitor reduced the palmitoylation of TF and increased TF oligomerization. A quadruple mutant of Cys33, Cys35, Cys41, and Cys43 in TF blocked its palmitoylation and reduced oligomerization. Furthermore, we determined the association of TF with nsP1 and nsP3 in a palmitoylation-dependent manner. Construction of intraviral interactome of CHIKV provides the basis for further studying the function of CHIKV proteins.