Odour-induced analgesia mediated by hypothalamic orexin neurons in mice.
Odour-induced analgesia mediated by hypothalamic orexin neurons in mice.
复制标题
小鼠下丘脑Orexin神经元介导的ODOR诱导的镇痛。
DOI：
10.1038/srep37129
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发表时间：
2016-11-15
影响因子：
4.6
通讯作者：
Kashiwadani H
中科院分区：
文献类型：
--
作者：
Tashiro S;Yamaguchi R;Ishikawa S;Sakurai T;Kajiya K;Kanmura Y;Kuwaki T;Kashiwadani H
各种民间疗法都使用某些具有镇痛作用的有气味的化合物。事实上，芳樟醇（一种在薰衣草提取物中发现的单萜醇）被发现可以通过皮下、腹膜内、鞘内和口服给药减轻疼痛反应。然而，嗅觉介导的有气味化合物的镇痛作用尚未得到彻底研究。我们对小鼠进行了在气味蒸气暴露下的行为疼痛测试。在检查的六种气味分子中，芳樟醇显着提高了疼痛阈值并减弱了疼痛行为。嗅球或上皮病变消除了这些影响，表明嗅觉感觉输入触发了这些影响。此外，免疫组织化学分析表明，芳樟醇激活下丘脑食欲素神经元，这是疼痛处理的关键介质之一。在食欲素神经元缺失和食欲素肽缺乏的小鼠中进行的福尔马林测试表明，食欲素能传递对于芳樟醇气味诱导的镇痛至关重要。总之，这些发现揭示了哺乳动物大脑中嗅觉输入触发的中枢镇痛回路，并支持通过芳樟醇气味刺激治疗疼痛的潜在治疗方法。
Various folk remedies employ certain odorous compounds with analgesic effects. In fact, linalool, a monoterpene alcohol found in lavender extracts, has been found to attenuate pain responses via subcutaneous, intraperitoneal, intrathecal, and oral administration. However, the analgesic effects of odorous compounds mediated by olfaction have not been thoroughly examined. We performed behavioural pain tests under odourant vapour exposure in mice. Among six odourant molecules examined, linalool significantly increased the pain threshold and attenuated pain behaviours. Olfactory bulb or epithelium lesion removed these effects, indicating that olfactory sensory input triggered the effects. Furthermore, immunohistochemical analysis revealed that linalool activated hypothalamic orexin neurons, one of the key mediators for pain processing. Formalin tests in orexin neuron-ablated and orexin peptide-deficient mice showed orexinergic transmission was essential for linalool odour-induced analgesia. Together, these findings reveal central analgesic circuits triggered by olfactory input in the mammalian brain and support a potential therapeutic approach for treating pain with linalool odour stimulation.