Site-specific integration of the actinophage R4 genome into the chromosome of Streptomyces parvulus upon lysogenization
Site-specific integration of the actinophage R4 genome into the chromosome of Streptomyces parvulus upon lysogenization
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溶原化后放线菌 R4 基因组位点特异性整合到小链霉菌染色体中
DOI：
10.1128/jb.173.13.4237-4239.1991
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发表时间：
1991
影响因子：
3.2
通讯作者：
H. Takahashi
中科院分区：
文献类型：
--
作者：
M. Shirai;H. Nara;A. Sato;T. Aida;H. Takahashi
放线菌噬菌体R4对小链霉菌的溶原作用是通过噬菌体基因组位点特异性整合到染色体中而发生的。对含有宿主染色体上的附着位点、噬菌体基因组和通过插入噬菌体基因组产生的两个连接点的DNA片段进行克隆和测序。发现附着位点共享12bp的共同核心。在S. coelicolor和S.青紫色。
The lysogenization of Streptomyces parvulus by actinophage R4 occurs by site-specific integration of the phage genome into the chromosome. The DNA fragments containing the attachment sites on the host chromosome, the phage genome, and the two junctions created by insertion of the phage genome were cloned and sequenced. The attachment sites were found to share a common core of 12 bp. This common core sequence was not detected in chromosomal DNAs of S. coelicolor and S. lividans.