Establishment of nerve growth factor gradients on aligned chitosan-polylactide /alginate fibers for neural tissue engineering applications.
Establishment of nerve growth factor gradients on aligned chitosan-polylactide /alginate fibers for neural tissue engineering applications.
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DOI：
10.1016/j.colsurfb.2017.10.017
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发表时间：
2017-12
期刊：
影响因子：
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通讯作者：
Hua Wu;Jiaoyan Liu;Qing Fang;Bo Xiao;Ying Wan
中科院分区：
文献类型：
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作者：
Hua Wu;Jiaoyan Liu;Qing Fang;Bo Xiao;Ying Wan
含有具有梯度分布的信号分子的对齐的纤维填充物的神经导管对于长间隙神经修复是必不可少的。本研究旨在开发一种在定向壳聚糖-聚乳酸（CH-PLA）纤维上构建神经生长因子（NGF）梯度的方法。使用湿纺技术将含有37重量%的PLA的CH-PLA纺成纤维。与壳聚糖纤维相比，CH-PLA纤维具有更高的湿态拉伸强度、更强的耐降解性和更低的溶胀度。选择直径为40 ~ 60 μm的CH-PLA纤维，用含NGF的海藻酸钠溶液将其分段成束涂覆，以沿纤维纵向沿着建立NGF梯度。所得负载NGF的CH-PLA/海藻酸盐纤维的直径被很好地控制在60 - 120 μm之间的范围内。纤维上的钙离子交联藻酸盐涂层显示出以梯度分布方式施用可持续的NGF释放至少5周的能力。NGF诱导的PC 12细胞突起生长证实了从纤维释放的NGF的生物活性被很好地保留。
Nerve conduits containing aligned fibrous fillers with gradiently distributed signal molecules are essential for long-gap nerve repair. This study was to develop an approach for establishing nerve growth factor (NGF) gradients onto the aligned chitosan-polylactide (CH-PLA) fibers. CH-PLA containing 37 wt% of PLA was spun into fibers using a wet-spinning technique. CH-PLA fibers showed much higher wet-state tensile strength, enhanced degradation tolerance and significantly lower swelling degree in comparison to chitosan fibers. The CH-PLA fibers with diameters from 40 to 60 μm were selected and segmentally coated in bundles using NGF-contained alginate solutions to establish NGF gradients lengthwise along fibers. The diameter of resulting NGF-loaded CH-PLA/alginate fibers was well controlled within a range between 60 and 120 μm. Calcium ion crosslinked alginate coating layers on fibers showed abilities to administer the sustainable NGF release in a gradient distribution manner for at least 5 weeks. NGF-induced neurite outgrowth of PC12 cells confirmed that bioactivity of NGF released from fibers was well retained.