Determination of bisphenols with estrogenic activity in plastic packaged baby food samples using solid-liquid extraction and clean-up with dispersive sorbents followed by gas chromatography tandem mass spectrometry analysis
Determination of bisphenols with estrogenic activity in plastic packaged baby food samples using solid-liquid extraction and clean-up with dispersive sorbents followed by gas chromatography tandem mass spectrometry analysis
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DOI：
10.1016/j.talanta.2017.09.067
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发表时间：
2018-02-01
期刊：
影响因子：
6.1
通讯作者：
Zafra-Gomez, A.
中科院分区：
文献类型：
--
作者：
Garcia-Corcoles, M. T.;Cipa, M.;Zafra-Gomez, A.
双酚(BPS)是一类已知具有内分泌干扰活性的化学物质。双酚A(BPA)是这类化学物质最具代表性的原型。最近，内分泌干扰物的原型双酚A的使用已经减少，取而代之的是结构类似物，因为它对环境和消费者都有负面影响。在这项工作中，提出了一种测定即食塑料包装婴儿食品中具有雌激素活性的7BPS的新方法。该程序包括用乙腈固-液相萃取分离分析物，然后用分散固相萃取剂(C18和PSA)和硫酸镁的混合物进行净化步骤，以减少蛋白质、糖和脂类对基质的影响。采用多元优化方法对提取工艺参数进行优化。用气相色谱-串联质谱仪(GC-MS/MS)对化合物进行检测和定量。对于所研究的分析物，定量限在0.1至1.2 ng g(-1)之间。用添加样品的矩阵匹配校正和回收率分析验证了方法的有效性。所有病例的回收率在91%~110%之间，%RSD<13%。该方法已成功地用于测定学龄前儿童食用的一种新型食物样本中的内分泌干扰物(EDCs)。这是第一次分析这一目标群体消费的塑料包装食品中的EDCs的研究。
Bisphenols (BPs) are a family of chemicals with known endocrine disrupting activity. Bisphenol A (BPA) is the most representative prototype of this group of chemicals. Recently, the use of BPA, a prototype of endocrine disruptors, has been reduced and replaced with structural analogs due to its negative effects on both the environment and consumers. In this work, a new method is presented for the determination of seven BPs, with estrogenic activity in ready-to-eat plastic packaged baby foods. The procedure involves the isolation of the analytes using solid-liquid phase extraction with acetonitrile followed by a clean-up step with a mixture of dispersive-SPE sorbents (C18 and PSA) and magnesium sulphate, to reduce matrix effect from proteins, sugars and lipids. Extraction parameters were optimized using multivariate optimization methods. The compounds were detected and quantified by gas chromatography tandem mass spectrometry (GC-MS/MS). The limits of quantification were between 0.1 and 1.2 ng g(-1) for the studied analytes. The method was validated using matrix-matched calibration and recovery assays with spiked samples. Recovery rates were between 91% and 110% and % RSD was lower than 13% in all cases. The method has been successfully applied for the determination of these endocrine disrupting chemicals (EDCs) in samples of a novel type of food consumed by pre-schoolers. This is the first study to analyze EDCs in plastic packaged foods consumed by this target group.