Reaction-based small-molecule fluorescent probes for chemoselective bioimaging.
Reaction-based small-molecule fluorescent probes for chemoselective bioimaging.
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构成生命基础的元素、离子和分子的动态化学多样性为研究提供了挑战和机会。小分子荧光探针可以利用选择性的生物正交化学来报告细胞和更复杂的生物样本中的特定分析物。这些探针提供了强大的试剂，以询问反应性化学物质在其天然环境中的生理学和病理学，对生命系统的干扰最小。这篇评论提出了一个调查的工具和策略，使用这样的探针来检测生物重要的化学分析物。我们强调设计标准，有效的化学工具，用于生物应用，以及未来的探索差距。
The dynamic chemical diversity of elements, ions and molecules that form the basis of life offers both a challenge and an opportunity for study. Small-molecule fluorescent probes can make use of selective, bioorthogonal chemistries to report on specific analytes in cells and in more complex biological specimens. These probes offer powerful reagents to interrogate the physiology and pathology of reactive chemical species in their native environments with minimal perturbation to living systems. This Review presents a survey of tools and tactics for using such probes to detect biologically important chemical analytes. We highlight design criteria for effective chemical tools for use in biological applications as well as gaps for future exploration.