CLUSEAN: A computer-based framework for the automated analysis of bacterial secondary metabolite biosynthetic gene clusters
CLUSEAN: A computer-based framework for the automated analysis of bacterial secondary metabolite biosynthetic gene clusters
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DOI：
10.1016/j.jbiotec.2009.01.007
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发表时间：
2009-03-10
影响因子：
4.1
通讯作者：
Wohlleben, W.
中科院分区：
文献类型：
--
作者：
Weber, T.;Rausch, C.;Wohlleben, W.
细菌次生代谢产物是抗菌药物和抗细胞药物的重要来源。这些分子通常是以多模块巨酶的方式逐步合成的，这些巨酶编码在编码酶的基因簇中，用于前体供应和修饰。在这项工作中，我们提出了一个开源软件流水线CLUSEAN(簇序列分析器)，它有助于注释和分析这类基因簇。CLUSEAN集成了标准的分析工具，如BLAST和HMMer，以及特定的工具来识别非核糖体肽合成酶(NRPS)/I型聚酮合成酶(PKS)的功能结构域和基序，并预测NRPS的特异性。(C)2009爱思唯尔B.V.保留所有权利。
Bacterial secondary metabolites are an important Source of antimicrobial and cytostatic drugs. These molecules are often synthesized in a stepwise fashion by multimodular megaenzymes that are encoded in Clusters of genes encoding enzymes for precursor supply and modification.In this work, we present an open Source software pipeline, CLUSEAN (CLUster SEquence ANalyzer) that helps to annotate and analyze such gene clusters. CLUSEAN integrates standard analysis tools, like BLAST and HMMer, with specific tools for the identification of the functional domains and motifs in nonribosomal peptide synthetases (NRPS)/type I polyketide synthases (PKS) and the prediction of specificities of NRPS. (C) 2009 Elsevier B.V. All rights reserved.