HIGH-GRADIENT MAGNETIC CELL-SEPARATION WITH MACS
HIGH-GRADIENT MAGNETIC CELL-SEPARATION WITH MACS
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DOI：
10.1002/cyto.990110203
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发表时间：
1990-01-01
期刊：
影响因子：
--
通讯作者：
RADBRUCH, A
中科院分区：
文献类型：
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作者：
MILTENYI, S;MULLER, W;RADBRUCH, A
开发并测试了一种灵活、快速、简单的磁性细胞分选系统，可根据特定的细胞表面标记分离大量细胞。用生物素化抗体、荧光染料偶联亲和素和超顺磁性生物素化微粒子（直径约100 nm）依次染色的细胞在高梯度磁柱上分离。未标记的细胞通过柱，而标记的细胞保留。保留的细胞可以很容易地被洗脱。在大约15分钟内可以处理超过109个细胞。富集率可达100倍以上，耗尽率可达1000倍以上。用荧光染料和非常小的不可见磁珠同时标记细胞使该系统成为流式细胞术的理想补充。细胞的光散射和荧光参数不受结合粒子的影响。磁分离细胞可以用荧光显微镜或流式细胞术进行分析，也可以用荧光活化细胞分选进行分类，而无需进一步处理。磁标记和分离不影响细胞活力和增殖。
A flexible, fast and simple magnetic cell sorting system for separation of large numbers of cells according to specific cell surface markers was developed and tested. Cells stained sequentially with biotinylated antibodies, fluorochrome-conjugated avidin, and superparamagnetic biotinylated-microparticles (about 100 nm diameter) are separated on high gradient magnetic (HGM) columns. Unlabelled cells pass through the column, while labelled cells are retained. The retained cells can be easily eluted. More than 109 cells can be processed in about 15 min. Enrichment rates of more than 100-fold and depletion rates of several 1,000-fold can be achieved. The simultaneous tagging of cells with fluorochromes and very small, invisible magnetic beads makes this system an ideal complement to flow cytometry. Light scatter and fluorescent parameters of the cells are not changed by the bound particles. Magnetically separated cells can be analysed by fluorescence microscopy or flow cytometry or sorted by fluorescence-activated cell sorting without further treatment. Magnetic tagging and separation does not affect cell viability and proliferation.