Functional FRIGIDA allele enhances drought tolerance by regulating the P5CS1 pathway in Arabidopsis thaliana
Functional FRIGIDA allele enhances drought tolerance by regulating the P5CS1 pathway in Arabidopsis thaliana
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功能性 FRIGIDA 等位基因通过调节拟南芥 P5CS1 途径增强耐旱性
DOI：
10.1016/j.bbrc.2017.11.149
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发表时间：
2018-01-01
影响因子：
3.1
通讯作者：
Kong, Xiangxiang
中科院分区：
文献类型：
--
作者：
Chen, Qian;Zheng, Yan;Kong, Xiangxiang
在适当的时间开花对于植物的繁殖成功和它们对环境胁迫的反应是重要的。在拟南芥中，开花时间的自然变异的主要决定因素是FRIGIDA（FRI）。在本研究中，我们表明，过表达的功能FRIGIDA基因在野生型Col背景（ColFRI）积极增强耐旱性激活P5CS1的表达，促进脯氨酸积累在水分胁迫。此外，FRI基因和蛋白表达水平没有观察到干旱处理的显着变化，而P5CS1蛋白表达显着增加。与此相反，春化处理有效地降低P5CS1的表达水平，并导致在ColFRI植物的耐旱性下降。具有功能性FRI背景的flc突变体在耐旱期间也减轻了FRI介导的P5CS1激活。综上所述，我们的研究结果揭示了FRI在水分亏缺胁迫下通过其下游P5CS1途径增强抗旱性的新功能，这取决于其靶基因FLC。(C)2017爱思唯尔公司All rights reserved.
Flowering at the right time is important for the reproductive success of plants and their response to environmental stress. In Arabidopsis, a major determinant of natural variation in flowering time is FRIGIDA (FRI). In the present study, we show that overexpression of the functional FRIGIDA gene in wild type Col background (ColFRI) positively enhances the drought tolerance by activating P5CS1 expression and promoting proline accumulation during water stress. Furthermore, no significant changes in FRI gene and protein expression levels were observed with drought treatment, whereas P5CS1 protein expression significantly increased. In contrast, vernalization treatment efficiently reduced P5CS1 expression levels and resulted in a decrease in drought tolerance in the ColFRI plants. The flc mutants with a functional FRI background also relieved FRI-mediated activation of P5CS1 during drought tolerance. Taken together, our findings reveal the novel function of FRI in enhancing drought resistance through its downstream P5CS1 pathway during water-deficit stress, which is dependent on its target, the FLC gene. (C) 2017 Elsevier Inc. All rights reserved.