Co-suppression of the EIN2-homology gene LeEIN2 inhibits fruit ripening and reduces ethylene sensitivity in tomato
Co-suppression of the EIN2-homology gene LeEIN2 inhibits fruit ripening and reduces ethylene sensitivity in tomato
复制标题
EIN2同源基因LeEIN2的共抑制抑制果实成熟并降低番茄乙烯敏感性
DOI：
10.1134/s102144371004014x
复制
发表时间：
2010-07-01
影响因子：
1.4
通讯作者：
Chen, G. P.
中科院分区：
文献类型：
--
作者：
Hu, Z. L.;Deng, L.;Chen, G. P.
EIN2（乙烯不敏感2）是乙烯信号转导途径中非常重要的组成部分。近日，我们实验室分离得到了LeEIN2（番茄EIN2）的基因组DNA和全长cDNA。为了揭示LeEIN2的功能，生产了LeEIN2表达水平降低的转基因番茄植物。与野生型 Ailsa Craig 相比，LeEIN2 沉默的转基因植物的果实成熟以及编码多聚半乳糖醛酸酶和 TomLoxB 的成熟相关基因的表达受到抑制。在幼苗乙烯反应测定中，LeEIN2表达减少的转基因番茄植株表现出乙烯不敏感性。这些结果表明LeEIN2在调节番茄果实成熟中发挥着关键作用，并且是乙烯信号转导途径的正调节因子。
EIN2 (ethylene insensitive 2) is a very important component in the ethylene signal transduction pathway. Recently, the genomic DNA and full-length cDNA of LeEIN2 (tomato EIN2) have been isolated in our laboratory. To reveal the function of LeEIN2, transgenic tomato plants with reduced expression levels of LeEIN2 were produced. The fruit ripening and expressions of ripening-related genes encoding polygalacturonase and TomLoxB were inhibited in the LeEIN2-silenced transgenic plants compared to the wild-type Ailsa Craig. In the seedling ethylene response assay, the transgenic tomato plants with reduced LeEIN2 expression exhibited ethylene insensitivity. These results indicate that LeEIN2 plays a critical role in regulating tomato fruit ripening and is a positive regulator in ethylene signal transduction pathway.