Optical coherence microscopy for deep tissue imaging of the cerebral cortex with intrinsic contrast
Optical coherence microscopy for deep tissue imaging of the cerebral cortex with intrinsic contrast
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DOI：
10.1364/oe.20.002220
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发表时间：
2012-01-30
期刊：
影响因子：
3.8
通讯作者：
Cable, Alex E.
中科院分区：
文献类型：
--
作者：
Srinivasan, Vivek J.;Radhakrishnan, Harsha;Cable, Alex E.
在体光学显微成像技术最近出现的神经生物学发展和病理生理学的研究的重要工具。特别是，双光子显微镜已被证明是一个强大的和高度灵活的方法，在体内成像的高散射组织。然而，双光子成像通常需要外源性染料或造影剂，并且成像深度限于几百微米。在这里，我们展示了光学相干显微镜（OCM）的神经元细胞体和皮质髓鞘形成的深度类似于1.3毫米的大鼠新皮层在体内成像。成像不需要施用外源性染料或造影剂，并且仅通过固有散射对比和图像处理来实现。此外，使用OCM，我们证明在体内，定量测量的光学特性（折射率和衰减系数）在皮质，并将这些属性与层状细胞结构确定的图像。最后，我们表明，OCM能够直接可视化细胞去极化过程中的细胞变化，因此可能提供新的光学标记的细胞活力。(C)2012美国光学学会
In vivo optical microscopic imaging techniques have recently emerged as important tools for the study of neurobiological development and pathophysiology. In particular, two-photon microscopy has proved to be a robust and highly flexible method for in vivo imaging in highly scattering tissue. However, two-photon imaging typically requires extrinsic dyes or contrast agents, and imaging depths are limited to a few hundred microns. Here we demonstrate Optical Coherence Microscopy (OCM) for in vivo imaging of neuronal cell bodies and cortical myelination up to depths of similar to 1.3 mm in the rat neocortex. Imaging does not require the administration of exogenous dyes or contrast agents, and is achieved through intrinsic scattering contrast and image processing alone. Furthermore, using OCM we demonstrate in vivo, quantitative measurements of optical properties (index of refraction and attenuation coefficient) in the cortex, and correlate these properties with laminar cellular architecture determined from the images. Lastly, we show that OCM enables direct visualization of cellular changes during cell depolarization and may therefore provide novel optical markers of cell viability. (C) 2012 Optical Society of America