Blood test detecting autoantibodies to N-methyl-D-aspartate neuroreceptors for evaluation of patients with transient ischemic attack and stroke
Blood test detecting autoantibodies to N-methyl-D-aspartate neuroreceptors for evaluation of patients with transient ischemic attack and stroke
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DOI：
10.1373/49.10.1752
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发表时间：
2003-10-01
影响因子：
9.3
通讯作者：
Skoromets, AA
中科院分区：
文献类型：
--
作者：
Dambinova, SA;Khounteev, GA;Skoromets, AA
背景：脑卒中是一种多系统疾病，包括血栓形成和神经毒性偶联机制。生化事件后分子级联的关键代谢物同时出现在脑组织、血脑屏障和脑血管中，激活免疫系统并产生针对脑特异性抗原的自身抗体（aAb）。我们开发了一种ELISA血液测试来测量针对N-甲基-D-天冬氨酸（NMDA）受体亚型的抗体，这是脑缺血神经毒性的关键标志物。我们研究了血清抗NR 2A/2B（NMDA受体亚型）抗体在评估短暂性脑缺血发作（TIA）和缺血性卒中（IS）中的诊断准确性及其区分脑缺血和脑出血（ICH）的能力。其中105例来自TIA/卒中患者，255例来自对照组，包括控制性高血压/动脉粥样硬化患者以及性别和年龄匹配的健康个体。TIA组（n = 56）和急性IS组（n = 31）的NR 2A/2B aAb浓度显著高于对照组（P
Background: Stroke is a multisystemic disorder that includes mechanisms of thrombosis and neurotoxic coupling. Key metabolites of the molecular cascade following biochemical events appear simultaneously in brain tissue, the blood-brain barrier, and brain vessels, activating the immune system and generating autoantibodies (aAbs) to brain-specific antigens. We developed an ELISA blood test to measure aAbs to a subtype of N-methyl-D-aspartate (NMDA) receptors, which are the key markers of neurotoxicity underlying cerebral ischemia. We investigated the diagnostic accuracy of serum aAbs to NR2A/2B, a subtype of NMDA receptors, in assessing transient ischemic attack (TIA) and ischemic stroke (IS) and its ability to distinguish cerebral ischemia from intracerebral hemorrhage (ICH).Methods: Autoantibodies to NR2A/2B were measured in 360 serum samples: 105 from TIA/stroke patients and 255 from controls, including patients with controlled hypertension/atherosclerosis and gender- and age-matched healthy individuals.Results: Patients with TIA (n = 56) and acute IS (n = 31) had significantly higher NR2A/2B aAb concentrations than controls (P