Validation of the drop coating deposition Raman method for protein analysis
Validation of the drop coating deposition Raman method for protein analysis
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DOI：
10.1016/j.ab.2006.03.025
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发表时间：
2006-06-15
影响因子：
2.9
通讯作者：
Ben-Amotz, Dor
中科院分区：
文献类型：
--
作者：
Ortiz, Corasi;Zhang, Dongmao;Ben-Amotz, Dor
对滴涂沉积拉曼（DCDR）光谱进行了严格评价，以确定其可用于检测蛋白质构象、结合和纯度变化的限度。差谱用于评估在各种实验条件下的DCDR光谱的再现性。结果表明：（i）在典型的DCDR数据收集条件下，不存在由拉曼激发激光引起的热/光化学激光损伤。(ii)不同体积或浓度样品的DCDR光谱的重现性。(iii)DCDR蛋白质沉积物的水含量和相关的光谱特征，以及（iv）溶液拉曼光谱和DCDR光谱之间的相似程度。(c)2006年爱思唯尔公司All rights reserved.
Drop coating deposition Raman (DCDR) spectroscopy is critically evaluated to establish the limits to which it may be used to detect changes in protein conformation, binding, and purity. Difference spectroscopy is used to evaluate the reproducibility of the DCDR spectra under various experimental conditions. The results indicate (i) the absence of thermal/photochemical laser damage induced by the Raman excitation laser under typical DCDR data collection conditions. (ii) the reproducibility of DCDR spectra from samples with different volumes or concentrations. (iii) the water content of DCDR protein deposits and associated spectral signatures, and (iv) the degree of similarity between solution Raman spectra and DCDR spectra. (c) 2006 Elsevier Inc. All rights reserved.