β-1,3-Glucan recognition protein 3 activates the prophenoloxidase system in response to bacterial infection in Ostrinia furnacalis Guenee
β-1,3-Glucan recognition protein 3 activates the prophenoloxidase system in response to bacterial infection in Ostrinia furnacalis Guenee
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DOI：
10.1016/j.dci.2017.10.004
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发表时间：
2018-02-01
影响因子：
2.9
通讯作者：
Feng, Congjing
中科院分区：
文献类型：
--
作者：
Wu, Taoyan;Zhao, Ya;Feng, Congjing
模式识别受体（PRRs）是结合非自身病原体相关分子模式（PAMPs）的生物传感器蛋白。β -1,3-葡聚糖识别蛋白（beta GRPs）在昆虫先天免疫的免疫识别和信号通路中发挥重要作用。本文报道了Ostrinia furnacalis幼虫Off β GRP3 cDNA的克隆与鉴定。Off beta GRP3包含1455 bp的开放阅读框，编码预测的484个氨基酸残基蛋白。在注射后2、4、8、10和12 h，圈杆菌攻毒组的血细胞中Off β GRP3的表达水平高于枯草芽孢杆菌攻毒组。在脂肪体中，枯草芽孢杆菌和大肠杆菌攻毒组Off β GRP3的表达在4 - 10 HPI范围内均显著高于未攻毒组，随后在12 - 36 HPI范围内持续下降。Ofi3GRP3在4 ~ 24 HPI各测试组织中注射层粘胶蛋白组的表达均高于注射脂多糖组。大肠杆菌感染的β GRP3-RNAi幼虫（1.0 d）的LT50显著低于野生型大肠杆菌感染的幼虫（3.0 d） （p < 0.01）。接种Off β GRP3 dsRNA的幼虫中，只有10.2%的Sephadex G50珠（3度）被完全黑化，而对照组的这一比例为48.8% （p < 0.01）。α - GRP3敲除幼虫血淋巴中PO活性和lease活性均显著降低。我们的研究表明，β - GRP3是参与ppo激活系统的PRR成员。furnacalis幼虫。(C) 2017 Elsevier Ltd.版权所有。
Pattern recognition receptors (PRRs) are biosensor proteins that bind to non-self pathogen associated molecular patterns (PAMPs). beta-1,3-glucan recognition proteins (beta GRPs) play an essential role in immune recognition and signaling pathway of insect innate immunity. Here, we report the cloning and characterization of cDNA of Off beta GRP3 from Ostrinia furnacalis larvae. The Off beta GRP3 contains 1455 bp open reading frame, encoding a predicted 484 amino acid residue protein. In hemocytes, the expression levels of Off beta GRP3 in Escherichia coil-challenged group were higher than those of Bacillus subtilis-challenged group at 2, 4, 8, 10 and 12 h post injection (HPI). In fat body, Off beta GRP3 expression in both B. subtilis and E. coli-challenged group was significantly higher than that in untreated group from 4 to 10 HPI, and then the expression continuously dropped from 12 to 36 HPI. The Ofi3GRP3 expression in laminarin-injected group was higher than that in lipopolysaccharides (LPS)-injected group in various test tissues from 4 to 24 HPI. The LT50 of E. coli-infected Of beta GRP3-RNAi larvae (1.0 days) was significantly lower compared with that of E. coli infected wild-type larvae (3.0 days) (p < 0.01). Only 10.2% Sephadex G50 beads (degree 3) were completely melanized in the larvae inoculated with Off beta GRP3 dsRNA, as compared to 48.8% in control larvae (p < 0.01). A notable reduction in the PO activity and lEARase activity in hemolymph was also detected in the Of beta GRP3 knockdown larvae. Our study demonstrates that Of beta GRP3 is one of PRR members involved the PPO-activating system in 0. furnacalis larvae. (C) 2017 Elsevier Ltd. All rights reserved.