Determination of tetracyclines by novel singlet-oxygen mediated cerium(IV) chemiluminescence
Determination of tetracyclines by novel singlet-oxygen mediated cerium(IV) chemiluminescence
复制标题
DOI：
10.1080/10739149.2016.1215995
复制
发表时间：
2017-03
影响因子：
1.6
通讯作者：
Chenyao Zhu;Lijun Wei;P. Yuan;Li-xian Xiong;Xianglei Cheng
中科院分区：
文献类型：
--
作者：
Chenyao Zhu;Lijun Wei;P. Yuan;Li-xian Xiong;Xianglei Cheng
摘要本文报道了在吐温80存在和不存在下，铈（IV）与5种四环素类药物的化学发光新机理。以2-甲基-6-（4-甲氧基苯基）-3，7-二氢咪唑并[1，2-a]吡嗪-3-酮盐酸盐为探针，用紫外光谱法研究了四环素类抗生素在该化学发光体系中的作用。这是第一次报道，铈（IV）与四环素反应，产生单线态氧1 O2后，打破有机骨架和分解的过氧乙酸在没有表面活性剂吐温。此外，通过考察叠氮化钠的影响和化学发光光谱，结果表明，在约480 nm处的最大化学发光发射是由于单线态氧1 O2的二聚体发射之间的铈（IV）和四环素以及铈（IV）和吐温80之间的反应，分别。此外，化学发光信号不受影响时，反应在厌氧条件下进行，表明溶解氧是独立的该系统。在最佳条件下，研究了铈（IV）-吐温80-四环素类化学发光体系的分析特性和参数。该方法已成功地用于牛奶中四环素类抗生素的测定。
ABSTRACT Novel chemiluminescence mechanisms of cerium(IV) and five tetracyclines in the absence and presence of Tween 80 are reported. The role of tetracyclines in the present chemiluminescence system was studied in detail by ultraviolet spectra and the singlet oxygen 1O2 probe 2-methyl-6-(4-methoxyphenyl)-3, 7-dihydroimidazo[1,2-a]-pyrazin-3-one hydrochloride. It is the first report that cerium(IV) reacted with tetracyclines producing singlet oxygen 1O2 after breaking the organic skeleton and decomposition of peroxyacetic acid in the absence of the surfactant Tween. Furthermore, by examining the effects of sodium azide and the chemiluminescence spectra, the results indicated that the maximum chemiluminescence emission at approximately 480 nm was due to dimer emission of singlet oxygen 1O2 from the reaction between cerium(IV) and tetracyclines as well as the reaction of cerium(IV) and Tween 80, respectively. Moreover, the chemiluminescence signals were unaffected when the reaction was performed under anaerobic condition, indicating that dissolved oxygen was independent of this system. Under the optimal conditions, the analytical characteristics and parameters of the cerium(IV)-Tween 80-tetracyclines chemiluminescence system were investigated. The present method was successfully employed for the determination of tetracyclines in milk.