Multilocus sequence typing (MLST) analysis of Propionibacterium acnes isolates from radical prostatectomy specimens.
Multilocus sequence typing (MLST) analysis of Propionibacterium acnes isolates from radical prostatectomy specimens.
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DOI：
10.1002/pros.22621
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发表时间：
2013-05
期刊：
影响因子：
2.8
通讯作者：
Sfanos, Karen S
中科院分区：
文献类型：
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作者：
Mak, Tim N;Yu, Shu-Han;De Marzo, Angelo M;Bruggemann, Holger;Sfanos, Karen S
炎症通常在根治性前列腺切除术标本中观察到，有证据表明炎症可能有助于前列腺癌的发生。多种微生物已被牵连作为刺激前列腺炎症。促炎厌氧菌痤疮丙酸杆菌（Propionibacterium acnes）在人类皮肤上普遍存在，并且与皮肤病寻常痤疮相关。最近的研究表明，痤疮丙酸杆菌可以通过细菌培养或非培养的分子技术在乳房切除术标本中检测到。从30名前列腺癌患者获得根治性前列腺切除术组织样品，并进行有氧和厌氧培养。通过16 S rDNA基因测序鉴定培养种。使用多位点序列分型（MLST）对痤疮丙酸杆菌分离株进行分型。我们的研究证实，痤疮丙酸杆菌可以很容易地培养从乳房切除术组织（7/30例，23%）。在某些情况下，培养痤疮丙酸杆菌的多个分离株以及其他丙酸杆菌属物种，如颗粒丙酸杆菌和嗜亲丙酸杆菌。总体而言，30例病例中有9例（30%）为丙酸杆菌属阳性。MLST分析在前列腺源性痤疮丙酸杆菌分离株中鉴定了8种不同的序列类型（ST）。这些ST属于两个克隆复合体，即CC 36（I-2型）和CC 53/60（II型），或者是CC 53/60相关的单例。MLST分型结果表明，前列腺源性痤疮丙酸杆菌分离株不属于典型的皮肤/痤疮ST，而是与机会性感染和/或尿道植物群相关的ST的特征。MLST分型结果反驳了来自皮肤植物群的痤疮丙酸杆菌分离株代表污染的可能性。
Inflammation is commonly observed in radical prostatectomy specimens, and evidence suggests that inflammation may contribute to prostate carcinogenesis. Multiple microorganisms have been implicated in serving as a stimulus for prostatic inflammation. The pro-inflammatory anaerobe, Propionibacterium acnes, is ubiquitously found on human skin and is associated with the skin disease acne vulgaris. Recent studies have shown that P. acnes can be detected in prostatectomy specimens by bacterial culture or by culture-independent molecular techniques. Radical prostatectomy tissue samples were obtained from 30 prostate cancer patients and subject to both aerobic and anaerobic culture. Cultured species were identified by 16S rDNA gene sequencing. Propionibacterium acnes isolates were typed using multilocus sequence typing (MLST). Our study confirmed that P. acnes can be readily cultured from prostatectomy tissues (7 of 30 cases, 23%). In some cases, multiple isolates of P. acnes were cultured as well as other Propionibacterium species, such as P. granulosum and P. avidum. Overall, 9 of 30 cases (30%) were positive for Propionibacterium spp. MLST analyses identified eight different sequence types (STs) among prostate-derived P. acnes isolates. These STs belong to two clonal complexes, namely CC36 (type I-2) and CC53/60 (type II), or are CC53/60-related singletons. MLST typing results indicated that prostate-derived P. acnes isolates do not fall within the typical skin/acne STs, but rather are characteristic of STs associated with opportunistic infections and/or urethral flora. The MLST typing results argue against the likelihood that prostatectomy-derived P. acnes isolates represent contamination from skin flora.