Compromised Blood Flow in the Optic Nerve Head after Systemic Administration of Aldosterone in Rats: A Possible Rat Model of Retinal Ganglion Cell Loss
Compromised Blood Flow in the Optic Nerve Head after Systemic Administration of Aldosterone in Rats: A Possible Rat Model of Retinal Ganglion Cell Loss
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DOI：
10.1080/02713683.2022.2029907
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发表时间：
2022-03-19
影响因子：
2
通讯作者：
Sugiyama, Kazuhisa
中科院分区：
文献类型：
--
作者：
Wada, Yasushi;Higashide, Tomomi;Sugiyama, Kazuhisa
目的观察醛固酮对大鼠视神经乳头（ONH）血流、视网膜血管直径及视网膜神经节细胞（RGC）的影响。方法在Brown Norway大鼠中使用渗透微型泵给予醛固酮（80 μ g/kg/天）或溶剂。在给予醛固酮或赋形剂之前和之后1、2和4周，通过激光散斑血流图测量ONH中血管（MV）和组织（MT）区域的平均模糊率以及视网膜血管直径。记录眼内压（IOP）、血压和心率。在治疗后5周制备的视网膜平片中计数逆行标记的RGC。结果醛固酮组MV和MT在2周和4周时均显著降低（MV：2周，p = 0.001，4周，p < 0.001; MT：2周，p = 0.02，4周，p = 0.03）。醛固酮组动脉和静脉直径在第1、2和4周显著减小（均P < 0.001）。溶剂组的MV、MT和血管直径保持不变。两组的其他参数均未随时间变化。醛固酮组的RGC计数显著低于载体组（p < 0.001）。结论在一种可能的通过全身给予醛固酮导致RGC丢失的大鼠模型中，视网膜血管收缩后ONH血流量减少，而IOP或血压没有变化。
Purpose To investigate the optic nerve head (ONH) blood flow, retinal vessel diameters, and retinal ganglion cell (RGC) loss after systemic administration of aldosterone in rats. Methods Aldosterone (80 mu g/kg/day) or vehicle was administered using an osmotic minipump in Brown Norway rats. The mean blur rate in the vessel (MV) and tissue (MT) regions and retinal vessel diameters in the ONH were measured by laser speckle flowgraphy before and 1, 2, and 4 weeks after administration of aldosterone or vehicle. Intraocular pressure (IOP), blood pressure, and heart rate were recorded. The retrogradely labeled RGCs were counted in the retinal flatmounts prepared 5 weeks after treatment. Results The MV and MT in the aldosterone group significantly decreased at 2 and 4 weeks (MV: 2 weeks, p = 0.001, 4 weeks, p < 0.001; MT: 2 weeks, p = 0.02, 4 weeks, p = 0.03). The artery and vein diameters significantly decreased at 1, 2, and 4 weeks in the aldosterone group (all p < 0.001). The MV, MT, and vessel diameters remained unchanged in the vehicle group. Other parameters did not change over time in either group. RGC counts were significantly lower in the aldosterone group than in the vehicle group (p < 0.001). Conclusions ONH blood flow decreased following retinal vessel constriction without changes in IOP or blood pressure in a possible rat model of RGC loss by systemic administration of aldosterone.