A novel structure of exopolysaccharide produced by a plant-derived lactic acid bacterium Lactobacillus paracasei IJH-SONE68
A novel structure of exopolysaccharide produced by a plant-derived lactic acid bacterium Lactobacillus paracasei IJH-SONE68
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DOI：
10.1093/jb/mvy048
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发表时间：
2018-08-01
影响因子：
2.7
通讯作者：
Sugiyama, Masanori
中科院分区：
文献类型：
--
作者：
Noda, Masafumi;Sugimoto, Sachiko;Sugiyama, Masanori
本课题组从无花果叶中分离到一株乳酸菌副乳杆菌IJH-SONE68，该菌能产生酸性和中性胞外多糖。核磁共振分析表明，前者主要由甘露糖组成，后者由N-乙酰氨基葡萄糖(GlcNAc)组成的α-1，6-连接糖链组成。α-1，6-连接的GlcNAc多糖的存在首次在原核生物中被报道。此外，为了揭示IJH-SONE68株EPS生物合成的基因组织，在本研究中，我们测定了全基因组序列。
A lactic acid bacterium Lactobacillus paracasei IJH-SONE68, which was isolated from a fig leaf by our group, was found to produce both acidic and neutral exopolysaccharides (EPSs). The nuclear magnetic resonance analysis demonstrates that the former EPS is composed primarily of mannose, and the latter one consists of the alpha-1, 6-linked glycan chains made of N-acetylglucosamine (GlcNAc). The presence of alpha-1, 6-linked GlcNAc polysaccharide is first reported in prokaryotes. Furthermore, to reveal the EPS-biosynthetic gene organization in the IJH-SONE68 strain, in the present study, we determined the whole-genome sequence.