PARASITE-ACCESSORY CELL-INTERACTIONS IN THEILERIOSIS - ANTIGEN PRESENTATION BY THEILERIA-ANNULATA-INFECTED MACROPHAGES AND PRODUCTION OF CONTINUOUSLY GROWING ANTIGEN-PRESENTING CELL-LINES
PARASITE-ACCESSORY CELL-INTERACTIONS IN THEILERIOSIS - ANTIGEN PRESENTATION BY THEILERIA-ANNULATA-INFECTED MACROPHAGES AND PRODUCTION OF CONTINUOUSLY GROWING ANTIGEN-PRESENTING CELL-LINES
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DOI：
10.1002/eji.1830201120
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发表时间：
1990-11-01
影响因子：
5.4
通讯作者：
SPOONER, RL
中科院分区：
文献类型：
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作者：
GLASS, EJ;SPOONER, RL
环形泰勒虫是牛的原生动物寄生虫，感染主要组织相容性复合体（MHC）II+类细胞，特别是巨噬细胞，并将其转化为持续生长的细胞系。我们研究了T. annulata对抗原呈递细胞功能的影响。T. annulata感染的细胞（TaH）将卵清蛋白（通过[3H]胸苷掺入测量）呈递给静息的自体牛T细胞和卵清蛋白特异性牛CD 4 + T细胞系。然而，前者的细胞也刺激TaH没有外源性抗原，虽然在较低程度上比抗原的存在。这种“非特异性”增殖在卵清蛋白特异性T细胞系中未观察到。静息T细胞在抗原存在下对TaH或纯化的外周血单核细胞的反应幅度基本相似。然而，在每个电池的基础上，需要较少的TaH。与单核细胞相比，在TaH存在下，卵清蛋白特异性T细胞系对抗原的增殖显著增加，并且再次需要更少的TaH来引起应答。处理的动力学似乎没有实质性改变TaH和增加的增殖可能是由于这些细胞的MHC II类表达升高。对T细胞系的遗传限制性研究表明，通过等电聚焦技术鉴定，TaH用于提呈卵清蛋白的限制元件与单核细胞使用的限制元件相同。不断增长的细胞系为我们提供了一个独特的模型，研究寄生虫辅助细胞相互作用泰勒虫病。TaH的抗原提呈细胞活性增强可能在疾病的发病机制中起重要作用。TaH也将为我们的抗原呈递研究提供宝贵的资源。特别是，TaH增强的抗原呈递使我们能够检测对较低水平抗原的反应，这通常是实验的重要考虑因素，其中可用抗原的量是主要限制因素。
Theileria annulata, a protozoan parasite of cattle, infects major histocompatibility complex (MHC) class II+ cells, particularly macrophages, and transforms them into continuously growing cell lines. We examined the effects of parasitism by T. annulata on antigen-presenting cell function. T. annulata infected cells (TaH) presented ovalbumin (as measured by [3H]thymidine incorporation) to both resting autologous bovine T-cells and ovalbumin-specific bovine CD4+ T cell lines. However, the former cells were also stimulated by TaH without exogenous antigen although to a lesser degree than in the presence of antigen. This "nonspecific" proliferation was not seen with the ovalbumin-specific T cell lines. The magnitude of response by resting T cells in the presence of antigen, to TaH or purified peripheral blood monocytes, was essentially similar. However, on a per cell basis fewer TaH were required. Considerably greater proliferation to antigen was seen with the ovalbumin-specific T cell lines in the presence of TaH compared to monocytes and again fewer TaH were required to elicit a response. The kinetics of processing did not appear to be substantially altered in TaH and the increased proliferation may be due to the elevated MHC class II expression of these cells. Genetic restriction studies with the T cell lines indicated that the restricting elements used to present ovalbumin by TaH were the same as those used by monocytes, as identified by an isoelectric focusing technique. The continuously growing cell lines provide us with a unique model for investigating parasite-accessory cell interactions in theileriosis. The augmented antigen presenting cell activity of TaH may play an important role in the pathogenesis of the disease. TaH will also provide us with a valuable resource for our antigen presentation studies. In particular, the enhanced antigen presentation by TaH enabled us to detect responses to lower levels of antigen, often an important consideration for experiments where the quantity of antigen available is the major limiting factor.