Calcium phosphate nanoparticles show an effective activation of the innate immune response in vitro and in vivo after functionalization with flagellin
Calcium phosphate nanoparticles show an effective activation of the innate immune response in vitro and in vivo after functionalization with flagellin
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DOI：
10.1007/s12250-014-3379-0
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发表时间：
2014-02
影响因子：
5.5
通讯作者：
Diana Kozlova;V. Sokolova;M. Zhong;E. Zhang;Jingyi Yang;Wei Li;Yi Yang;J. Buer;A. Westendorf-A.-Weste
中科院分区：
文献类型：
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作者：
Diana Kozlova;V. Sokolova;M. Zhong;E. Zhang;Jingyi Yang;Wei Li;Yi Yang;J. Buer;A. Westendorf-A.-Weste
对于亚单位疫苗，佐剂在形成靶向抗原特异性免疫应答的幅度、持久性和形式方面发挥关键作用。鞭毛蛋白是一种有效的免疫激活剂，通过桥接先天性炎症反应和适应性免疫，并且是临床应用的候选佐剂。磷酸钙纳米颗粒是不同生物分子如DNA、RNA、肽和蛋白质的有效载体。制备了鞭毛蛋白功能化的磷酸钙纳米颗粒，并研究了其对天然免疫系统的免疫刺激作用，即细胞因子的产生。它们在体外诱导促炎细胞因子IL-8（Caco-2细胞）和IL-1β（骨髓源性巨噬细胞; BMDM）的产生，在小鼠腹腔注射后在体内诱导IL-6的产生。免疫刺激比游离鞭毛蛋白更明显。
For subunit vaccines, adjuvants play a key role in shaping the magnitude, persistence and form of targeted antigen-specific immune response. Flagellin is a potent immune activator by bridging innate inflammatory responses and adaptive immunity and an adjuvant candidate for clinical application. Calcium phosphate nanoparticles are efficient carriers for different biomolecules like DNA, RNA, peptides and proteins. Flagellin-functionalized calcium phosphate nanoparticles were prepared and their immunostimulatory effect on the innate immune system, i.e. the cytokine production, was studied. They induced the production of the proinflammatory cytokines IL-8 (Caco-2 cells) and IL-1β (bone marrow-derived macrophages; BMDM)in vitroand IL-6in vivoafter intraperitoneal injection in mice. The immunostimulation was more pronounced than with free flagellin.