Identification of the earliest B lineage stage in mouse bone marrow
Identification of the earliest B lineage stage in mouse bone marrow
复制标题
DOI：
10.1016/s1074-7613(00)80268-x
复制
发表时间：
1996-12-01
期刊：
影响因子：
32.4
通讯作者：
Hardy, RR
中科院分区：
文献类型：
--
作者：
Li, YS;Wasserman, R;Hardy, RR
我们在小鼠骨髓的 CD45R (B220)(+)CD24 (HSA)(-) 前原 B 部分中鉴定了非常早期阶段的 B 谱系细胞，其通过 AA4.1 的表达进行描述，AA4.1 是一种在干细胞和早期 B 谱系细胞上发现的分子。这些细胞是 B 谱系前体细胞，因为它们能够在基质依赖性培养中快速生成 B 谱系细胞，并且在没有 Rag-1/2 的情况下表达高水平的种系 IgH 转录本。这些 AA4.1(+) 前体中有一半表达低水平的 CD4，这是淋巴祖细胞的特征，但很少有上调 CD19（一种在 B 谱系中非常早期表达的分子）。此外，编码前 B 和 B 细胞受体成分（mb-1、B29 和 lambda 5）的基因以及 B 谱系分化所需的转录因子（BSAP、E12、E47 和 Id）的表达为将这些细胞指定为最早的 B 细胞前体提供了进一步的支持。
We have identified a very early stage of B lineage cells in the CD45R (B220)(+)CD24 (HSA)(-) pre-pro-B fraction of mouse bone marrow delineated by expression of AA4.1, a molecule found on stem cells and early B lineage cells. These cells are B lineage precursors based on their capacity to generate B lineage cells rapidly in stromal-dependent culture and their expression of high levels of germline IgH transcripts in the absence of Rag-1/2. Half of these AA4.1(+) precursors express low levels of CD4, characteristic of lymphoid progenitors, but few if any have up-regulated CD19, a molecule expressed very early in the B lineage. Furthermore, expression of genes encoding pre-B and B cell receptor components (mb-1, B29, and lambda 5) and transcription factors necessary for B lineage differentiation (BSAP, E12, E47, and Id) provide further support for designating these cells as the earliest B cell precursors.