The Zuker collection:: A resource for the analysis of autosomal gene function in Drosophila melanogaster
The Zuker collection:: A resource for the analysis of autosomal gene function in Drosophila melanogaster
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DOI：
10.1534/genetics.167.1.203
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发表时间：
2004-05-01
期刊：
影响因子：
3.3
通讯作者：
Becker, AH
中科院分区：
文献类型：
--
作者：
Koundakjian, EJ;Cowan, DM;Becker, AH
多细胞生物的大多数基因编码的蛋白质的功能不是生存所需的，但有助于重要的生理功能，如行为和生殖。据估计，黑腹果蝇的基因中有75%是非必需的。在这里，我们报告的战略，用于建立一个大的收集股票，是适合于恢复这些基因的突变。从差不多72,000架F-3。分离常染色体的培养物用甲磺酸乙酯（EMS）大量处理，类似于12，000个株系，其中处理的第二或第三染色体在纯合条件下存活。EMS剂量诱导的突变率估计为1.2-1.5 × 10 ~（-3）个/基因，预测在6000条第二染色体和6000条第三染色体的样本中，每条染色体有5 - 6个非必需基因突变，每个位点有7 - 9个等位基因。由于在初始暴露于诱变剂时诱导的嵌合突变，许多品系分离或现在固定为诱变染色体上的致死突变。这个被称为Zuker集合的集合的特征使其成为影响广泛生物功能的突变的正向和反向遗传筛选的宝贵资源。
The majority of genes of multicellular organisms encode proteins with functions that are not required for viability but contribute to important physiological functions such as behavior and reproduction. It is estimated that 75% of the genes of Drosophila melanogaster are nonessential. Here we report on a strategy used to establish a large collection of stocks that is suitable for the recovery of mutations in such genes. From similar to72,000 F-3. cultures segregating for autosomes heavily treated with ethyl methanesulfonate (EMS), similar to12,000 lines in which the treated second or third chromosome survived in homozygous condition were selected. The dose of EMS induced an estimated rate of 1.2-1.5 X 10(-3) mutations/gene and predicts five to six nonessential gene mutations per chromosome and seven to nine alleles per locus in the samples of 6000 second chromosomes and 6000 third chromosomes. Due to mosaic mutations induced in the initial exposure to the mutagen, many of the lines are segregating or are now fixed for lethal mutations on the mutagenized chromosome. The features of this collection, known as the Zuker collection, make it a valuable resource for forward and reverse genetic screens for mutations affecting a wide array of biological functions.