Widespread capacity for steroid synthesis in the avian brain and song system
Widespread capacity for steroid synthesis in the avian brain and song system
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DOI：
10.1210/en.2006-0154
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发表时间：
2006-12-01
期刊：
影响因子：
4.8
通讯作者：
Schlinger, Barney A.
中科院分区：
文献类型：
--
作者：
London, Sarah E.;Monks, D. Ashley;Schlinger, Barney A.
类固醇对许多物种的大脑和行为都有强大的影响，但对鸣禽内分泌生理的测量和操作往往揭示了类固醇和大脑之间无法解释的联系。斑马雀鸣叫系统是终生对类固醇敏感的感觉运动神经回路，其组织和功能在很大程度上明显独立于性腺衍生的类固醇。我们测试了一种假设，即斑马雀的大脑具有从头合成类固醇的能力，神经合成的类固醇可能会影响歌唱系统。我们利用多种技术证明类固醇合成途径中的前三个因子--类固醇生成急性调节蛋白(STAR)、细胞色素P450侧链裂解(Cyp11a1)和3β-羟基类固醇脱氢酶/Delta 5-Delta 4异构酶在发育中和成年斑雀脑中都有表达。在后20天(P20)和成人的详细表达图谱显示，类固醇生成急性调节蛋白Cyp11a1和3β-羟基类固醇脱氢酶/Delta 5-Delta 4异构酶广泛的区域特异性表达和共表达模式，这表明神经类固醇可能调节包括感觉和运动系统在内的多种大脑功能。值得注意的是，尽管其他类固醇生成基因如芳香酶的表达在SONG系统中基本上是缺失的，但每个主要的SONG核至少表达这里所述的类固醇生成基因的一个子集，从而将SONG系统确立为潜在的类固醇生成回路。
Steroids exert powerful effects on the brains and behavior of many species, but measures and manipulations of endocrine physiology in songbirds often reveal unexplained connections between steroids and the brain. The zebra finch song system, a sensorimotor neural circuit sensitive to steroids throughout life, organizes and functions largely in apparent independence from gonadally derived steroids. We tested the hypothesis that the zebra finch brain has the capacity for de novo steroidogenesis and that neurally synthesized steroids, neurosteroids, may impact the song system. Using multiple techniques, we demonstrate that the steroidogenic acute regulatory protein (StAR), cytochrome P450 side-chain cleavage (CYP11A1), and 3 beta-hydroxysteroid dehydrogenase/Delta 5-Delta 4 isomerase, the first three factors in the steroidogenic pathway, are expressed in both developing and adult zebra finch brain. Detailed expression mapping at posthatch d 20 (P20) and adult reveals widespread area-specific expression and coexpression patterns for steroidogenic acute regulatory protein, CYP11A1, and 3 beta-hydroxysteroid dehydrogenase/Delta 5-Delta 4 isomerase, which suggest neurosteroids may modulate multiple brain functions, including sensory and motor systems. Notably, whereas expression of other steroidogenic genes such as aromatase has been essentially absent from the song system, each of the major song nuclei express at least a subset of steroidogenic genes described here, establishing the song system as a potential steroidogenic circuit.