Transformation of HBCDs by Rhodococcus sp. stu-38
Transformation of HBCDs by Rhodococcus sp. stu-38
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DOI：
10.1007/s00284-022-02872-y
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发表时间：
2022-05
影响因子：
2.6
通讯作者：
Fei Yu;Wenqi Luo;Yuyang Li;S. Meng;Xianbin Lin;Lele Li;Xueying Ye;Hui Wang;Tao Peng
中科院分区：
文献类型：
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作者：
Fei Yu;Wenqi Luo;Yuyang Li;S. Meng;Xianbin Lin;Lele Li;Xueying Ye;Hui Wang;Tao Peng
1,2,5,6,9,10-六溴环十二烷（hbcd）是一种严重污染环境的溴化阻燃剂。环境中的hbcd可以转化为各种产品。转化产物的鉴定已经使用各种质谱技术进行。然而，细菌转化hbcd产生低水平产物的研究并不充分。本文分离得到了能在无机盐培养基、海水和生长培养基中立体选择性转化hbcd的arhodococcusstu -38菌株。采用GC-MS鉴定了7种潜在的hbcd生物转化产物。这些产品包括溴化烯烃、二溴环十二烯和溴环十二烯；溴化烯醇、溴环十二烯醇和溴环十二烯醇；全溴化化合物环十二烯二醇、1,2-环氧-5,9-环十二烯二烯和环十二烯二醇含量较低，可能导致假阴性结果。低水平转化产物的毒性评价较少，不容忽视。本研究重点鉴定了低水平转化产物，揭示了hbcd复杂的立体选择性生物转化过程。
1,2,5,6,9,10-Hexabromocyclododecanes (HBCDs) are brominated flame retardants causing serious environmental pollution. HBCDs in the environment could be transformed to various products. Identification of transformation products has been performed using various mass-spectrometric techniques. However, bacterial transformation of HBCDs yielding low-level products was not well studied. In this paper, aRhodococcusstrain stu-38 which could stereoselectively transform HBCDs in mineral salt medium, seawater, and growth medium was isolated. Seven potential biotransformation products of HBCDs were identified by using GC–MS. These products, including brominated alkenes, dibromocyclododecadiene and bromocyclododecatriene; brominated alkenols, bromocyclododecadienol and bromocyclododecatrienol; fully debrominated compounds, cyclododecadiendiol, 1,2-epoxy-5,9-cyclododecadiene, and cyclododecadienol, were presented in rather low level which could lead to false negative results. The low-level transformation products should not be ignored because their toxicity was less assessment. This research highlighted identification of the low-level transformation products to reveal the complicated stereoselective biotransformation of HBCDs.