Correlation of F4-neuroprostanes levels in cerebrospinal fluid with outcome of aneurysmal subarachnoid hemorrhage in humans
Correlation of F4-neuroprostanes levels in cerebrospinal fluid with outcome of aneurysmal subarachnoid hemorrhage in humans
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DOI：
10.1016/j.freeradbiomed.2009.06.026
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发表时间：
2009-09-15
影响因子：
7.4
通讯作者：
Yen, Hsiu-Chuan
中科院分区：
文献类型：
--
作者：
Hsieh, Yu-Ping;Lin, Chih-Lung;Yen, Hsiu-Chuan
动脉瘤性蛛网膜下腔出血（aSAH）是人类出血性卒中的一种类型。F-2-异前列烷（F-2-IsoPs）和F-4-神经前列烷（F-4-NPs）分别来源于花生四烯酸和二十二碳六烯酸（DHA），是脂质过氧化的特异性标志物。我们先前证明aSAH患者脑脊液（CSF）中的F-2-IsoPs水平与不良临床状况呈正相关。在这项工作中，我们改进了F-4-NPs分析，并通过检测CSF中的F-4-NPs研究了aSAH患者神经元潜在氧化损伤的作用。使用[H-2（4）]-15-F-2 t-IsoP而不是[O-18（2）]-17-F-4c-NP或[H-2（4）]-PGF（2 α）作为F-4-NP分析的内标物。使用[O-18（2）]-17-F-4c-NP的一个问题是CSF中F-2-二高-IsoPs产生的潜在干扰。分析了15例aSAH患者长达10天的CSF标本和12例非aSAH对照的CSF标本。aSAH患者F-4-NP的第一天、平均值和峰值水平均显著高于对照组，并与Fisher量表和3个月格拉斯哥结局量表相关，但只有F-4-NP的平均水平与Hunt和Hess分级相关。结果首先证明了aSAH后脑组织中DHA的氧化损伤，并表明在早期时间点，CSF中的F-4-NP可能是比F-2-IsoPs更好的aSAH结果的预测因子。(C)2009 Elsevier Inc. All rights reserved.
Aneurysmal subarachnoid hemorrhage (aSAH) is one type of hemorrhagic stroke in humans. F-2-isoprostanes (F-2-IsoPs) and F-4-neuroprostanes (F-4-NPs), derived from arachidonic acid and docosahexaenoic acid (DHA), respectively, are specific markers of lipid peroxidation. We previously demonstrated that F-2-IsoPs levels in cerebrospinal fluid (CSF) of aSAH patients positively correlated with poor clinical conditions. In this work, we refined F-4-NPs analysis and investigated the role of potential oxidative damage to neurons in aSAH patients by detecting F-4-NPs in CSF. [H-2(4)]-15-F-2t-IsoP, rather than [O-18(2)]-17-F-4c-NP or [H-2(4)]-PGF(2 alpha), was used as the internal standard for F-4-NPs analysis. One problem of the use of [O-18(2)]-17-F-4c-NP was the potential interference resulting from F-2-dihomo-IsoPs in CSF. CSF specimens of 15 aSAH patients for up to 10 days and those of 12 non-aSAH controls were analyzed. First day, mean, and peak levels of F-4-NPs were all significantly higher in aSAH patients than in controls and correlated with the Fisher Scale and 3-month Glasgow Outcome Scale, but only mean levels of F-4-NPs correlated with Hunt and Hess Grade. The results first demonstrate oxidative damage to DHA in brain tissue following aSAH and suggest that F-4-NPs in CSF could be a better predictor for outcome of aSAH than F-2-IsoPs at early time points. (C) 2009 Elsevier Inc. All rights reserved.