Fasciola hepatica Gastrodermal Cells Selectively Release Extracellular Vesicles via a Novel Atypical Secretory Mechanism.
Fasciola hepatica Gastrodermal Cells Selectively Release Extracellular Vesicles via a Novel Atypical Secretory Mechanism.
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肝片形吸虫胃真皮细胞通过一种新的非典型分泌机制选择性释放细胞外囊泡。
DOI：
10.3390/ijms23105525
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发表时间：
2022-05-15
影响因子：
5.6
通讯作者：
中科院分区：
文献类型：
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作者：
肝片形吸虫是专性供血者，寄生虫的胃胚层细胞形成与宿主血液的界面。尽管它们在宿主-寄生虫相互作用中的重要性，但缺乏对胃胚层细胞的深入蛋白质组学分析。在这里，我们使用激光显微切割F。肝脏组织切片，以生成胃真皮细胞和外皮的独特且生物学上排他性的组织部分，用于质谱分析。共鉴定出226种胃胚层细胞蛋白质，其中降解血红蛋白的蛋白酶最丰富。其他检测到的蛋白质包括质子泵和抗凝剂等，它们维持了促进消化的微环境。通过比较胃胚层细胞蛋白质组和102种蛋白质在激光显微切割的皮层与以前发表的皮层蛋白质组数据集，我们发现，四分之一的蛋白质（通过冻融提取去除）或三分之一的蛋白质（通过洗涤剂提取去除）以前确定为皮层，而不是来自胃胚层细胞。比较分析了激光显微切割的胃胚叶细胞、被膜和F。hepatica分泌组的研究表明，胃胚层细胞是分泌蛋白的主要来源，并且表明胃胚层细胞和被膜都可能释放细胞外囊泡（EV）亚群。从宿主胆管中取出后立即固定的吸虫肠盲囊的显微镜检查表明，所选的胃上皮细胞经历了顶端质膜的逐渐变薄，其破裂以释放分泌囊泡进入肠腔。我们的研究结果表明，肠源性EV是通过一种新的非典型分泌途径释放的，并强调了胃胚层细胞在营养获取和寄生虫可能的免疫调节中的重要性。
The liver fluke, Fasciola hepatica, is an obligate blood-feeder, and the gastrodermal cells of the parasite form the interface with the host’s blood. Despite their importance in the host–parasite interaction, in-depth proteomic analysis of the gastrodermal cells is lacking. Here, we used laser microdissection of F. hepatica tissue sections to generate unique and biologically exclusive tissue fractions of the gastrodermal cells and tegument for analysis by mass spectrometry. A total of 226 gastrodermal cell proteins were identified, with proteases that degrade haemoglobin being the most abundant. Other detected proteins included those such as proton pumps and anticoagulants which maintain a microenvironment that facilitates digestion. By comparing the gastrodermal cell proteome and the 102 proteins identified in the laser microdissected tegument with previously published tegument proteomic datasets, we showed that one-quarter of proteins (removed by freeze–thaw extraction) or one-third of proteins (removed by detergent extraction) previously identified as tegumental were instead derived from the gastrodermal cells. Comparative analysis of the laser microdissected gastrodermal cells, tegument, and F. hepatica secretome revealed that the gastrodermal cells are the principal source of secreted proteins, as well as showed that both the gastrodermal cells and the tegument are likely to release subpopulations of extracellular vesicles (EVs). Microscopical examination of the gut caeca from flukes fixed immediately after their removal from the host bile ducts showed that selected gastrodermal cells underwent a progressive thinning of the apical plasma membrane which ruptured to release secretory vesicles en masse into the gut lumen. Our findings suggest that gut-derived EVs are released via a novel atypical secretory route and highlight the importance of the gastrodermal cells in nutrient acquisition and possible immunomodulation by the parasite.
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影响因子：
5.7
作者：
Herron CM;O'Connor A;Robb E;McCammick E;Hill C;Marks NJ;Robinson MW;Maule AG;McVeigh P
通讯作者：
McVeigh P
影响因子：
4
作者：
Cameron, Timothy C.;Cooke, Ira;Spithill, Terry W.
通讯作者：
Spithill, Terry W.
影响因子：
3.3
作者：
Fontao, L;Favre, B;Borradori, L
通讯作者：
Borradori, L
DOI：
10.1074/mcp.m115.053934
发表时间：
2015-12
期刊：
Molecular & cellular proteomics : MCP
影响因子：
--
作者：
Cwiklinski K;de la Torre-Escudero E;Trelis M;Bernal D;Dufresne PJ;Brennan GP;O'Neill S;Tort J;Paterson S;Marcilla A;Dalton JP;Robinson MW
通讯作者：
Robinson MW
影响因子：
4.8
作者：
Collins, PR;Stack, CM;Donnelly, S
通讯作者：
Donnelly, S