Imaging Mitochondrial Dynamics in the Xenopus Central Nervous System (CNS).
Imaging Mitochondrial Dynamics in the Xenopus Central Nervous System (CNS).
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DOI：
10.1101/pdb.prot106807
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发表时间：
2021-04-01
影响因子：
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通讯作者：
Bestman JE
中科院分区：
文献类型：
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作者：
Feng MS;Bestman JE
线粒体以通过氧化磷酸化产生 ATP 而闻名，还控制钙稳态、脂肪生成、活性氧的调节和细胞凋亡。即使在相对简单的细胞内，线粒体在形状、丰度、运动和亚细胞位置方面也是异质的。它们以相互连接的管状网络和运动细胞器的形式存在，沿着细胞骨架运输以分布在整个细胞中。这些空间和形态特征反映了细胞器合成 ATP 和支持细胞的能力的变异性。线粒体的变化被认为支持细胞功能和命运，而线粒体功能障碍是神经系统疾病的基础。在这里，我们描述了一种体内延时成像方法，用于监测和测量发育中的白化爪蟾蝌蚪脑细胞中线粒体的运动和位置。使用非洲爪蟾进行这些实验的无与伦比的好处是，可以在体内测量细胞中线粒体形态和分布的测量，其中周围的神经回路和影响这些关键细胞器的其他输入保持完整。
Notable for producing ATP via oxidative phosphorylation, mitochondria also control calcium homeostasis, lipogenesis, the regulation of reactive oxygen species, and apoptosis. Even within relatively simple cells, mitochondria are heterogeneous with regard to their shape, abundance, movement and subcellular locations. They exist as interconnected, tubular networks, and as motile organelles that are transported along the cytoskeleton to distribute throughout cells. These spatial and morphological features reflect the variability in the organelle’s capacity to synthesize ATP and support the cells. Changes to mitochondria are believed to support cell function and fate, and mitochondrial dysfunction underlies disease in the nervous system. Here we describe an in vivo timelapse imaging approach to monitor and measure the movement and position of the mitochondria in cells of the developing brain albino Xenopus laevis tadpoles. The unparalleled benefit for using Xenopus for these experiments is that measurements of mitochondrial morphology and distribution in cells can be measured in vivo, where the surrounding neural circuitry and other inputs that influence these critical organelles remain intact.