Lab-On-A-Chip Device for Yeast Cell Characterization in Low-Conductivity Media Combining Cytometry and Bio-Impedance
Lab-On-A-Chip Device for Yeast Cell Characterization in Low-Conductivity Media Combining Cytometry and Bio-Impedance
复制标题
DOI：
10.3390/s19153366
复制
发表时间：
2019-08-01
期刊：
影响因子：
3.9
通讯作者：
Kourtiche, Djilali
中科院分区：
文献类型：
--
作者：
Claudel, Julien;De Araujo, Arthur Luiz Alves;Kourtiche, Djilali
本文提出了一种简单的方法来优化工作频带的芯片上的实验室的生物阻抗细胞术为基础的单细胞。它主要涉及在低电导率介质中的应用。生物阻抗允许通过提供电特征来表征低细胞浓度或单细胞。因此，可能需要执行高达几十兆赫的阻抗测量，以便提取内部细胞特征。在单细胞的情况下，在低至1 pL的非常小的体积中进行表征。同时，测量的阻抗从生理液体的几十千欧姆增加到低电导率介质的几兆欧姆。例如，水分析就是这种情况。在频率高于数百千赫兹时，寄生效应（如耦合电容）可能会超过样品的阻抗，并使测量完全短路。为了在这些条件下优化传感器，开发了细胞仪装置的完整模型，包括传感器的寄生耦合电容以考虑所有阻抗。看来，它是可能的，以增加通带通过优化轨道的几何形状和布局，而不改变感测区域。这一假设是通过测量和比较酵母细胞在低电导率介质（自来水）中的电特性得到的。与以前的非优化传感器相比，获得了高于10倍的耦合电容降低，该传感器允许在低电导率介质中对小至酵母细胞的细胞的所有电特性进行阻抗测量。
This paper proposes a simple approach to optimize the operating frequency band of a lab-on-a-chip based on bio-impedance cytometry for a single cell. It mainly concerns applications in low-conductivity media. Bio-impedance allows for the characterization of low cell concentration or single cells by providing an electrical signature. Thus, it may be necessary to perform impedance measurements up to several tens of megahertz in order to extract the internal cell signature. In the case of single cells, characterization is performed in a very small volume down to 1 pL. At the same time, measured impedances increase from tens of kilo-ohms for physiological liquids up to several mega-ohms for low conductivity media. This is, for example, the case for water analysis. At frequencies above hundreds of kilohertz, parasitic effects, such as coupling capacitances, can prevail over the impedance of the sample and completely short-circuit measurements. To optimize the sensor under these conditions, a complete model of a cytometry device was developed, including parasitic coupling capacitances of the sensor to take into account all the impedances. It appears that it is possible to increase the pass band by optimizing track geometries and placement without changing the sensing area. This assumption was obtained by measuring and comparing electrical properties of yeast cells in a low-conductivity medium (tap water). Decreased coupling capacitance by a factor higher than 10 was obtained compared with a previous non-optimized sensor, which allowed for the impedance measurement of all electrical properties of cells as small as yeast cells in a low-conductivity medium.